RFLP linkage analysis and mapping genes controlling the fatty acid profile of Brassica juncea using reciprocal DH populations
2003
Mahmood, T. | Ekuere, U. | Yeh, F. | Good, A. G. | Stringam, G. R.
An RFLP linkage map, comprising 300 linked and 16 unlinked loci, was constructed using reciprocal DH populations of Brassica juncea. The linked loci were organized into 18 linkage groups and seven unlinked segments, covering a total map distance of 1,564 cM. The A and B genomes were identified. The χ² test showed that 96.1% of the common intervals in the two populations differed non-significantly for recombination fractions, thus strongly suggesting the absence of sex-based differences for recombination fractions in B. juncea. Two QTLs, E₁ₐ and E₁b, significantly affected erucic acid content, and individually explained 53.7% and 32.1%, respectively, and collectively 85.8% of the phenotypic variation in the population. The QTLs E₁ₐ and E₁b showed epistasis, and the full model including epistasis explained nearly all of the phenotypic variation in the population. The QTLs E₁ₐ and E₁b were also associated with contents of oleic, linoleic and linolenic acids. Three additional QTLs (LN₂, LN₃ and LN₄) significantly influenced linolenic acid content. The QTL LN₂ accounted for 35.4% of the phenotypic variation in the population. Epistatic interactions were observed between the QTLs E1a and LN₂. The stability of the detected QTLs across years and locations, and breeding strategies for improving the fatty acid profile of B. juncea, are discussed.
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