Heterokaryosis and vegetative compatibility in Leptographium wageneri
1990
Zambino, P.J. | Harrington, T.C.
Vegetative compatibility was tested among 76 isolates of Leptographium wageneri representing three taxonomic varieties and 14 electrophoretic phenotypes (i.e., unique combinations of isozyme patterns of 21 enzymes). Nitrate non-utilizing mutants selected on media containing the nitrate analogue chlorate were paired on nitrate media containing 200 ppm Triton X-100. The development of dense hyphal growth in the zone of confrontation between complementing phenotypes indicated compatibility. Heterokaryons were recovered from hyphal tips and conidiophores of complementing pairings but not from single conidia. Fourteen groups of vegetatively compatible isolates (VC groups) were detected among the three varieties. Each VC group contained isolates of similar electrophoretic phenotype, and most VC groups had unique geographic ranges. No complementation occurred between varieties. The relatively low number of VC groups and the correlations of VC groups with electrophoretic phenotype and geographic distributions indicate low diversity in L. wageneri, perhaps due to clonal selection, founder effects, and a lack of recombination.
اظهر المزيد [+] اقل [-]الكلمات المفتاحية الخاصة بالمكنز الزراعي (أجروفوك)
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