Follistatin alters myostatin gene expression in C<sub>2</sub>C<sub>12</sub> muscle cells
2004
Kocams¸, H. | Gulmez, N. | Aslan, S. | Nazlı, M.
The objective of the present study was to determine the effects of follistatin addition on myostatin and follistatin gene expression patterns in C<sub>2</sub>C<sub>12</sub> muscle cells. C<sub>2</sub>C<sub>12</sub> cells were administered with 100 ng/ml recombinant human (rh) follistatin in Dulbecco's modified Eagle medium (DMEM) containing 10% fetal bovine serum (FBS), 4 mM glutamine and antibiotics daily for three days. Rh follistatin was not added in the control wells. Follistatin and myostatin gene cDNAs were synthesised by reverse transcriptase polymerase chain reaction (RT-PCR).The time course of follistatin gene expression pattern was similar in both the control and the follistatin-treated group. Myostatin mRNA level significantly increased in the follistatin-treated group after 24 h of culture (Fig. 3, P < 0.01). Amounts then sharply decreased (Fig. 3, P < 0.01) at 48 h of culture, whereas there was no significant difference between the control and the follistatin-treated group at 72 h of culture. Our results demonstrated that myostatin and follistatin mRNA were expressed in C<sub>2</sub>C<sub>12</sub> cells and rh follistatin changed the myostatin expression pattern.
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