Using DNA Barcoding to Identify the Genus <i>Lolium</i>
2015
Shenmao, WU | YIN, Liping | DENG, Zhirui | CHEN, Qin | Yining, FU | XUE, Huajie
Seeds of the genus <em>Lolium</em> are difficult to identify based on morphology for morphological likeness and some physical deformation such as friction and flattening during storage and transport. DNA barcoding, a newly-established method, has been used to discriminate a variety of agricultural crops with its own advantages. In present study, DNA barcodes for the genus <em>Lolium</em> were investigated for the first time. DNA sequences of <em>psbA-trnH</em>,<em> rbcL</em>,<em> atpF-atpH</em>, and the <em>ITS2</em> region were evaluated for their ability to differentiate <em>Lolium</em> from the related genus <em>Festuca</em>. As confirmed by inter-intraspecific divergence and Kimura 2 parameter analysis, the greatest divergence existed in <em>ITS2</em>, followed by psbA-trnH. On the contrary, <em>rbcL</em> and <em>atpF-atpH</em> possessed poor genetic variation of 0-0.0115, and was relatively difficult in discrimination of genus <em>Lolium</em>. For <em>ITS2</em> sequence, no inter-intraspecific distance overlaps were observed and each species has a distinct barcoding gap. <em>ITS2</em> could effectively discriminate all species based on a neighbor-joining tree. Thus, the <em>ITS2</em> region is a candidate for DNA barcoding of <em>Lolium</em>.
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