Multiplication of Chrysanthemum shoots in bioreactors as affected by culture method and inoculation density of single node stems
2005
Hahn, E.J. | Paek, K.Y.
Single node cuttings (1 cm in length) of Chrysanthemum were cultured on gelled and liquid media to compare shoot multiplication efficiency. Liquid culture resulted in greater fresh weight, dry weight, shoot length and leaf area compared to gelled culture. Shoots from liquid culture grew vigorously without hyperhydricity, showing 100% ex vitro survival. To determine optimal inoculation density of single nodes in a bioreactor, different numbers of single nodes (20 or 40 or 60 or 80) were placed into a 10-l column-type bioreactor. Shoot length was greatest at the 80-node inoculation, with the least number of branches, indicating the best inoculation density tested for shoot multiplication in bioreactors. In the final experiment, single-node cuttings in bioreactors were treated with three different culture systems: ebb and flood, deep flow technique (DFT) culture and immersion. Results indicated that the DFT culture led to the greatest fresh weight, shoot length and leaf area, followed by the ebb and flood culture, while the immersion culture suppressed shoot multiplication due to the lack of oxygen and the high water potential. Our results suggested the possibility of large-scale production of Chrysanthemum shoots in bioreactors.
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