Biochemical basis of resistance to phenylamide fungicides
1990
Davidse, L.C.
Phenylamide fungicides selectively inhibit ribosomal RNA synthesis of sensitive Oomycete fungi by interference with the activity of the RNA polymerase I-template complex. Phenylamides bind to an as yet unidentified component of this complex. The phenylamide binding site in resistant strains has a very low affinity for metalaxyl, whereas values of the dissociation constant are in the order of 0.1-0.2 micromolar for phenylamidesensitive strains. Apparently a change at the phenylamide receptor is responsible for resistance. Identification of the phenylamide receptor is hampered by loss of its ability to bind phenylamides during purification. To overcome this a photoaffinity probe for the phenylamide receptor has been synthesized. The biological properties of this probe allow its use to tag the components of the enzyme complex involved in binding.
اظهر المزيد [+] اقل [-]الكلمات المفتاحية الخاصة بالمكنز الزراعي (أجروفوك)
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