EFFECT OF COMBINED SALT STRESS OF ALKALINE TYPE ON BIOLOGICAL INDICATORS, ANTIOXIDANT AND PROOXIDANT ENZYME SYSTEMS OF SOYBEAN SPROUTS

Ziyaddin Mammadov; Naila Aliyeva; Leyla Karimova Nigar Shahbazi

EFFECT OF COMBINED SALT STRESS OF ALKALINE TYPE ON BIOLOGICAL INDICATORS, ANTIOXIDANT AND PROOXIDANT ENZYME SYSTEMS OF SOYBEAN SPROUTS

2023

Ziyaddin Mammadov | Naila Aliyeva | Leyla Karimova Nigar Shahbazi

The main goal of this research was to investigate the changes in the biological indicators, antioxidant and pro-antioxydant enzyme systems of 10-day-old soybean sprouts under stress conditions created by the equimolar concentration of NaHCO3 + Na2 CO3 salts. The results of the experiments revealed that the created stress has a serious negative effect on the development of the root and leaf systems of seedlings, including the amount of chlorophyll in the leaves. This effect begins to manifest itself at the weakest applied concentration and becomes stronger as the concentration of salts increases. At 150 mM concentration, the mass of the root system decreases by 3.5 times, and that of the leaves by about 2 times, compared to the control. A similar result is also observed in the dry weight of the root and leaf system of seedlings. The low concentration of salts (50 mM) induced the activity of antioxidant enzymes in the tissues of the root system of seedlings, while in the leaves it had a weak inhibitory effect on the activity of SOD and GR enzymes, and a stimulating effect on the activity of APX and CAT enzymes. Due to salt increase in the habitat, SOD and GP were weakened in both tissue cells, while in APX and CAT enzymes, this situation was observed only at the highest concentration of salt solution.

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