Untargeted peptidomics approach of sheep and goat infant formulas submitted to in vitro digestions

This conference is a major event in the field of Food, Nutrition and Health.It is organized in the frame of the INFOGEST research network(www.cost-infogest.eu) whose objective is to “improve the health properties of food by sharing our knowledge on the digestive process”. INFOGESTis a global network of approximately 582 research scientists (academicand food companies) from 150 institutions across 46 countries.​

International audience

إنجليزي. Background: Untargeted peptidomics are well established holistic disciplines enabling researchers to characterize functional characteristics of foods at a molecular level. The aim of this work was to employ a comparative peptidomics approach to identify and measure relative quantities of sheep and goat formula milk peptides after in vitro gastrointestinal digestion. Method: Goat and sheep infant formulas were submitted to dynamic in vitro gastro-intestinal digestions using the DIDGI® system adapted to the newborn digestive conditions. High resolution nanoLC mass spectrometry analysis allowed the comparison of the kinetic of peptides released during digestions. Results and Conclusions: Peptides deriving from osteopontin-1, amylopid A and lactotransferrin, GlyCAM1 were characteristics of sheep and goat formula milk, respectively. Additionally, 280 and 330 peptides were already present in sheep and goat milk before ingestion, indicating that proteolysis events occurred in milk during formula transformationand storage processes. Furthermore, milk peptides were also checked for their unique functional properties, such as anti-hypertensive, immunomodulatory and prebiotic activities. Comparison of the peptides released from two proteins of animal origin in the context of infantdigestion could give more knowledge using alternative protein sources as cow milk protein remain the main source in commercial infant formulas.