Transcriptome versus Genomic Microsatellite Markers: Highly Informative Multiplexes for Genotyping Abies alba Mill. and Congeneric Species
2014
Postolache, Dragos | Leonarduzzi, Cristina | Piotti, Andrea | Spanu, Ilaria | Roig, Anne | Fady, Bruno | Roschanski, Anna | Liepelt, Sascha | Vendramin, Giovanni Giuseppe | Plant Genetics Institute ; National Research Council of Italy | Consiglio Nazionale delle Ricerche (CNR) | Scuola Universitaria Superiore Sant'Anna = Sant'Anna School of Advanced Studies [Pisa] (SSSUP) | Università degli studi di Parma = University of Parma (UNIPR) | Ecologie des Forêts Méditerranéennes (URFM) ; Institut National de la Recherche Agronomique (INRA) | Faculty of Biology, Conservation Biology ; Philipps Universität Marburg = Philipps University of Marburg | Italian MIUR project "Biodiversitaly" [RBAP10A2T4]; ERA-Net BiodivERsA LinkTree project [EUI2008-03713] | ANR-12-EBID-0003,Tiptree,Scénarios pour la dynamique de la biodiversité des forêts européennes sous changement global identifier les points de basculement micro-evolutifs(2012) | ANR-08-BDVA-0006,LINK TREE,Relations entre diversité génétique et réponse écologique aux changements environementaux : les arbres forestiers comme modèles d'étude(2008)
International audience
اظهر المزيد [+] اقل [-]إنجليزي. The availability of high-resolution, cost-effective polymorphic genetic markers displaying Mendelian inheritance is a prerequisite for fine-scale population genetic analyses as well as informed conservation and sustainable management. Silver fir (Abies alba Mill.) is a widespread European species of economic and ecological importance for which genetic markers are needed but difficult to develop, as in most conifer species. In this work, we introduce two sets of new multiplexed transcriptome-derived expressed sequence tag microsatellites (EST-simple sequence repeats (SSRs)) which we compare to a set of multiplexed genomic microsatellites (gSSRs). For both marker types, transferability was tested in 17 congeneric taxa. A total of 16 new EST-SSRs and two new gSSRs were developed. The EST-SSR multiplexes produced easily scorable amplification patterns that allow rapid and cost-effective genotyping at low-error rates, and include loci that display very low null allele frequencies. Generally, EST-SSRs displayed lower polymorphism and frequency of null alleles, but higher genetic differentiation among populations than gSSRs. Preliminary tests revealed that the EST-SSR markers are highly transferable and polymorphic across Abies species. This study also confirmed that SSRs can be successfully developed using next-generation sequencing technology also in large genome species such as conifers.
اظهر المزيد [+] اقل [-]الكلمات المفتاحية الخاصة بالمكنز الزراعي (أجروفوك)
المعلومات البيبليوغرافية
تم تزويد هذا السجل من قبل Institut national de la recherche agronomique