IDENTIFICATION OF EIGHT HALOTOLERANT STREPTO-MYCETE ISOLATES USING A SUGGESTED NUMERICAL TAXONOMY
2005
H. Mohamed Sonya, | Saleh E.A. | Zaki M.M.
A numerical method was suggested for the identification of eight local halotolerant streptomycete isolates. Cultural, morphological, physiological and antagonistic characteristics of those isolates were determined. Arbitrary scoring of these characters for the eight unknown isolates and those of the more related known Streptomyces species in the keyof Pridham and Tresner (1974) was given and resulted 58 characteristic units. The degree of similarity was determined using the Dice coefficient method and clustering was achieved using the unweighted pair group method average (UPGMA) algorithm. Using this numerical method, six out of the eight Streptomyces isolates, i.e., ST08, ST09, ST11, ST13, ST14 and ST15 were identified as S. longisporus, S. janthinus, S. griseochromogenes, S. antibioticus S. baarnensis and S. albolongus with 100, 95.8, 95.0, 92.8, 95.0 and 91.1%, respectively. Isolates ST10 and ST12 were duplicate of S. echinatus with similarities of 93.3 and 94.6%, respectively. Application of the suggested numerical taxonomy on the 14 known Streptomyces species revealed that these species fell into three major clusters based on their color of aerial mycelia.
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