Genetic Mapping, Candidate Gene Identification and Marker Validation for Host Plant Resistance to the Race 4 of Fusarium oxysporum f. sp. cubense Using Musa acuminata ssp. malaccensis
2023
Chen, Andrew | Sun, Jiaman | Viljoen, Altus | Mostert, Diane | Xie, Yucong | Mangila, Leroy | Bothma, Sheryl | Lyons, Rebecca | Hřibová, Eva | Christelová, Pavla | Uwimana, Brigitte | Amah, Delphine | Pearce, Stephen | Chen, Ning | Batley, Jacqueline | Edwards, David | Doležel, Jaroslav | Crisp, Peter | Brown, Allan, F. | Martin, Guillaume | Yahiaoui, Nabila | D'Hont, Angélique | Coin, Lachlan | Swennen, Rony | Aitken, Elizabeth, A. B. | School of Agriculture and Food Sciences ; University of Southern Queensland (USQ) | JiaYing University | Stellenbosch University | Duke University [Durham] | Institute of Experimental Botany of the Czech Academy of Sciences (IEB / CAS) ; Czech Academy of Sciences [Prague] (CAS) | International Institute of Tropical Agriculture (IITA Uganda) ; International Institute of Tropical Agriculture [Nigeria] (IITA) ; Consultative Group on International Agricultural Research [CGIAR] (CGIAR)-Consultative Group on International Agricultural Research [CGIAR] (CGIAR) | International Institute of Tropical Agriculture [Nigeria] (IITA) ; Consultative Group on International Agricultural Research [CGIAR] (CGIAR) | Rothamsted Research ; Biotechnology and Biological Sciences Research Council (BBSRC) | The University of Western Australia (UWA) | International Institute of Tropical Agriculture (IITA-Tanzania) ; International Institute of Tropical Agriculture [Nigeria] (IITA) ; Consultative Group on International Agricultural Research [CGIAR] (CGIAR)-Consultative Group on International Agricultural Research [CGIAR] (CGIAR) | Amélioration génétique et adaptation des plantes méditerranéennes et tropicales (UMR AGAP) ; Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Institut Agro Montpellier ; Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Université de Montpellier (UM) | Département Systèmes Biologiques (Cirad-BIOS) ; Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad) | The Peter Doherty Institute for Infection and Immunity [Melbourne] ; University of Melbourne-The Royal Melbourne Hospital | Catholic University of Leuven = Katholieke Universiteit Leuven (KU Leuven) | Laboratory of Tropical Crop Improvement ; Catholic University of Leuven = Katholieke Universiteit Leuven (KU Leuven) | This research was funded by the Bill and Melinda Gates Foundation, grant number (OPP1213871), and the APC was funded by (OPP1213871/INV-002979). This work was supported, in part, by the Bill and Melinda Gates Foundation (OPP1213871) through a program with the International Institute of Tropical Agriculture. Research funding support was also provided, in part, by Hort Innovation Australia (BA 17006) and by Bayer Crop Science through a technical assistance grant Acceleration of the identification of the resistance genes in banana and the generation of markers for the banana breeding program'. Jiaman Sun is supported by Guangxi Science and Technology Planning Project (Guike AA21196005) and Guangxi Natural Science Foundation (2021GXNSFAA196014). Under the grant conditions of the foundation, a Creative Commons Attribution 4.0 Generic License has already been assigned to the author-accepted manuscript version that might arise from this submission.
International audience
اظهر المزيد [+] اقل [-]إنجليزي. Fusarium wilt of banana is a devastating disease that has decimated banana production worldwide. Host resistance to Fusarium oxysporum f. sp. Cubense (Foc), the causal agent of this disease, is genetically dissected in this study using two Musa acuminata ssp. Malaccensis segregating populations, segregating for Foc Tropical (TR4) and Subtropical (STR4) race 4 resistance. Marker loci and trait association using 11 SNP-based PCR markers allowed the candidate region to be delimited to a 12.9 cM genetic interval corresponding to a 959 kb region on chromosome 3 of ‘DH-Pahang’ reference assembly v4. Within this region, there was a cluster of pattern recognition receptors, namely leucine-rich repeat ectodomain containing receptor-like protein kinases, cysteine-rich cell-wall-associated protein kinases, and leaf rust 10 disease-resistance locus receptor-like proteins, positioned in an interspersed arrangement. Their transcript levels were rapidly upregulated in the resistant progenies but not in the susceptible F2 progenies at the onset of infection. This suggests that one or several of these genes may control resistance at this locus. To confirm the segregation of single-gene resistance, we generated an inter-cross between the resistant parent ‘Ma850’ and a susceptible line ‘Ma848’, to show that the STR4 resistance co-segregated with marker ‘28820’ at this locus. Finally, an informative SNP marker 29730 allowed the locus-specific resistance to be assessed in a collection of diploid and polyploid banana plants. Of the 60 lines screened, 22 lines were predicted to carry resistance at this locus, including lines known to be TR4-resistant, such as ‘Pahang’, ‘SH-3362’, ‘SH-3217’, ‘Ma-ITC0250’, and ‘DH-Pahang/CIRAD 930’. Additional screening in the International Institute for Tropical Agriculture’s collection suggests that the dominant allele is common among the elite ‘Matooke’ NARITA hybrids, as well as in other triploid or tetraploid hybrids derived from East African highland bananas. Fine mapping and candidate gene identification will allow characterization of molecular mechanisms underlying the TR4 resistance. The markers developed in this study can now aid the marker-assisted selection of TR4 resistance in breeding programs around the world.
اظهر المزيد [+] اقل [-]الكلمات المفتاحية الخاصة بالمكنز الزراعي (أجروفوك)
المعلومات البيبليوغرافية
تم تزويد هذا السجل من قبل Institut national de la recherche agronomique