Mixed-Linkage Glucan Is the Main Carbohydrate Source and Starch Is an Alternative Source during Brachypodium Grain Germination
2023
Francin-Allami, Mathilde | Bouder, Axelle | Geairon, Audrey | Alvarado, Camille | Le-Bot, Lucie | Daniel, Sylviane | Shao, Mingqin | Laudencia-Chingcuanco, Debbie | Vogel, John | Guillon, Fabienne | Bonnin, Estelle | Saulnier, Luc | Sibout, Richard | Unité de recherche sur les Biopolymères, Interactions Assemblages (BIA) ; Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE) | BIBS - Plateforme Bioressources : Imagerie, Biochimie & Structure ; Unité de recherche sur les Biopolymères, Interactions Assemblages (BIA) ; Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-INRAE, PROBE Research Infrastructure | Joint Genome Institute (JGI) ; United States Department of Energy | USDA-ARS Western Regional Research Center | DOE Joint Genome Institute [Walnut Creek] | Département Aliments, produits biosourcés et déchets - INRAE (TRANSFORM) ; Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE) | The work (proposal: 10.46936/10.25585/60001041) conducted by the U.S. Department of Energy Joint Genome Institute (https://ror.org/04xm1d337), a DOE Office of Science User Facility, is supported by the Office of Science of the U.S. Department of Energy operated under Contract No. DE-AC02-05CH11231. INRAE
International audience
اظهر المزيد [+] اقل [-]إنجليزي. Seeds of the model grass Brachypodium distachyon are unusual because they contain very little starch and high levels of mixed-linkage glucan (MLG) accumulated in thick cell walls. It was suggested that MLG might supplement starch as a storage carbohydrate and may be mobilised during germination. In this work, we observed massive degradation of MLG during germination in both endosperm and nucellar epidermis. The enzymes responsible for the MLG degradation were identified in germinated grains and characterized using heterologous expression. By using mutants targeting MLG biosynthesis genes, we showed that the expression level of genes coding for MLG and starch-degrading enzymes was modified in the germinated grains of knocked-out cslf6 mutants depleted in MLG but with higher starch content. Our results suggest a substrate-dependent regulation of the storage sugars during germination. These overall results demonstrated the function of MLG as the main carbohydrate source during germination of Brachypodium grain. More astonishingly, cslf6 Brachypodium mutants are able to adapt their metabolism to the lack of MLG by modifying the energy source for germination and the expression of genes dedicated for its use.
اظهر المزيد [+] اقل [-]الكلمات المفتاحية الخاصة بالمكنز الزراعي (أجروفوك)
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تم تزويد هذا السجل من قبل Institut national de la recherche agronomique