Detection of foot-and-mouth disease viruses from the A/AFRICA/G-I genotype in the Sultanate of Oman
2024
Al-Rawahi, Wafa Ahmed | Elshafie, Elshafie Ibrahim | Baqir, Senan | Al-Ansari, Aliya | Wadsworth, Jemma | Hicks, Hayley | Knowles, Nick | Di Nardo, Antonello | King, Donald P. | Zientara, Stephan | Al Salloom, Fajer | Sangula, Abraham | Bernelin-Cottet, Cindy | Bakkali, Labib | Al Riyami, Bahja | Sultan Qaboos University (SQU) | The Pirbright Institute ; Biotechnology and Biological Sciences Research Council (BBSRC) | Virologie UMR1161 (VIRO) ; École nationale vétérinaire d'Alfort (ENVA)-Laboratoire de santé animale, sites de Maisons-Alfort et de Normandie ; Agence nationale de sécurité sanitaire de l'alimentation, de l'environnement et du travail (ANSES)-Agence nationale de sécurité sanitaire de l'alimentation, de l'environnement et du travail (ANSES)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE) | Ministry of Municipalities Affairs & Agriculture (MUN) | National Foot and Mouth Disease Laboratory, Nairobi, Kenya
International audience
اظهر المزيد [+] اقل [-]إنجليزي. Rapid identification and characterization of circulating foot-and-mouth disease virus (FMDV) strains is crucial for effective disease control. In Oman, a few serological and molecular studies have been conducted to identify the strains of FMDV responsible for the outbreaks that have been occurring within the country. In this study, 13 oral epithelial tissue samples from cattle were collected from suspected cases of FMD in Ash Sharqiyah North, Al Batinah North, Dhofar and Ad Dhakhyilia governorates of Oman between 2018 and 2021. FMDV RNA was detected in all samples by real-time RT-PCR and viruses were isolated after one- or two-blind passages in the porcine Instituto Biologico-Rim Suino-2 cell line. Antigen capture ELISA characterized all isolates as serotype A and VP1 phylogenetic analysis placed all sequences within a single clade of the G-I genotype within the A/AFRICA topotype. These sequences shared the closest nucleotide identities to viruses circulating in Bahrain in 2021 (93.5% to 99.5%) and Kenya in 2017 (93.4% to 99.1%). To the best of our knowledge, this is the first time that A/AFRICA/G-I viruses have been detected in Oman. Together with the closely related viruses detected recently in Bahrain, these findings reinforce the importance of deploying effective quarantine control measures to minimize the risks of transboundary transmission of FMD associated with the importation of cattle from East Africa.
اظهر المزيد [+] اقل [-]الكلمات المفتاحية الخاصة بالمكنز الزراعي (أجروفوك)
المعلومات البيبليوغرافية
تم تزويد هذا السجل من قبل Institut national de la recherche agronomique