Target enrichment sequencing coupled with GWAS identifies MdPRX10 as a candidate gene in the control of budbreak in apple
2024
Watson, Amy, E. | Guitton, Baptiste | Soriano, Alexandre | Rivallan, Ronan | Vignes, Hélène | Farrera, Isabelle | Huettel, Bruno | Arnaiz, Catalina | Falavigna, Vítor, da Silveira | Coupel-Ledru, Aude | Segura, Vincent | Sarah, Gautier | Dufayard, Jean-François | Sidibé-Bocs, Stéphanie | Costes, Evelyne | Andrés, Fernando | Amélioration génétique et adaptation des plantes méditerranéennes et tropicales (UMR AGAP) ; Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Institut Agro Montpellier ; Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Université de Montpellier (UM) | Département Systèmes Biologiques (Cirad-BIOS) ; Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad) | South Green Bioinformatics Platform [Montpellier] | Max Planck-Genome-centre Cologne (MP-GC) ; Max-Planck-Gesellschaft | Max Planck Institute for Plant Breeding Research (MPIPZ) | Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria = National Institute for Agricultural and Food Research and Technology (INIA) | Centro de Biotecnología y Genómica de Plantas ; Universidad Politécnica de Madrid-Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria (INIA) | The author(s) declare financial support was received for the research, authorship, and/or publication of this article. This project received funding from ERA-NET SusCrop2 (FruitFlow, ANR-21-SUSC-0002) to support the postdoctoral post of AEW and from the Agropolis Foundation (ID 1503-008) through the Investissements d'Avenir program (Labex Agro: ANR-10-LABX-0001) for capture sequencing. | ANR-21-SUSC-0002,FruitFlow,Predicting and tuning seasonal responses of apple and peach to improve orchard yield and climate change resilience(2021) | ANR-10-LABX-0001,AGRO,Agricultural Sciences for sustainable Development(2010)
International audience
اظهر المزيد [+] اقل [-]إنجليزي. The timing of floral budbreak in apple has a significant effect on fruit production and quality. Budbreak occurs as a result of a complex molecular mechanism that relies on accurate integration of external environmental cues, principally temperature. In the pursuit of understanding this mechanism, especially with respect to aiding adaptation to climate change, a QTL at the top of linkage group (LG) 9 has been identified by many studies on budbreak, but the genes underlying it remain elusive. Here, together with a dessert apple core collection of 239 cultivars, we used a targeted capture sequencing approach to increase SNP resolution in apple orthologues of known or suspected A. thaliana flowering time-related genes, as well as approximately 200 genes within the LG9 QTL interval. This increased the 275 223 SNP Axiom (R) Apple 480 K array dataset by an additional 40 857 markers. Robust GWAS analyses identified MdPRX10, a peroxidase superfamily gene, as a strong candidate that demonstrated a dormancy-related expression pattern and down-regulation in response to chilling. In-silico analyses also predicted the residue change resulting from the SNP allele associated with late budbreak could alter protein conformation and likely function. Late budbreak cultivars homozygous for this SNP allele also showed significantly up-regulated expression of C-REPEAT BINDING FACTOR (CBF) genes, which are involved in cold tolerance and perception, compared to reference cultivars, such as Gala. Taken together, these results indicate a role for MdPRX10 in budbreak, potentially via redox-mediated signaling and CBF gene regulation. Moving forward, this provides a focus for developing our understanding of the effects of temperature on flowering time and how redox processes may influence integration of external cues in dormancy pathways.
اظهر المزيد [+] اقل [-]الكلمات المفتاحية الخاصة بالمكنز الزراعي (أجروفوك)
المعلومات البيبليوغرافية
تم تزويد هذا السجل من قبل Institut national de la recherche agronomique