Investigating apical adverse effects of four endocrine active substances in the freshwater gastropod Lymnaea stagnalis
2014
Giusti, Arnaud | Lagadic, Laurent | Barsi, Alpar | Thome, J. P. | Joaquim-Justo, C. | Ducrot, Virginie | Écologie et santé des écosystèmes (ESE) ; Institut National de la Recherche Agronomique (INRA)-AGROCAMPUS OUEST | Laboratory of Animal Ecology and Ecotoxicology, Centre of Analytical Research and Technology (CART) ; Université de Liège = University of Liège = Universiteit van Luik = Universität Lüttich (ULiège)
The hermaphroditic gastropod Lymnaea stagnalis is proposed as a candidate species for the development of OECD guidelines for testing of the reprotoxicity of chemicals, including endocrine active substances (EASs). Up to now, only a few putative EASs have been tested for their reproductive toxicity in this species. In this study, we investigate the effects of four EASs with different affinities to the vertebrate estrogen and androgen receptors (chlordecone as an estrogen; cyproterone acetate, fenitrothion and vinclozolin as anti-androgens) on the reproduction of L stagnalis in a 21-day semi-static test. Testosterone and 17 alpha-ethinylestradiol (EE2) were used as the reference compounds. The tested EASs had no significant effect on growth and survival at the tested concentration ranges (ng to mu g/L. Classical reproduction endpoints (i.e., oviposition and fecundity) were not responsive to the tested chemicals, except for chlordecone and 17 alpha-ethinylestradiol, which hampered reproduction from 19.6 mu g/L and 17.6 mu g/L, respectively. The frequency of polyembryonic eggs, used as an additional endpoint, demonstrated the effects of all compounds except EE2. The molecular pathways, which are involved in such reproduction impairments, remain unknown. Our results suggest that egg quality is a more sensitive endpoint as compared to other reproductive endpoints commonly assessed in mollusk toxicity tests. (C) 2014 Elsevier B.V. All rights reserved.
اظهر المزيد [+] اقل [-]الكلمات المفتاحية الخاصة بالمكنز الزراعي (أجروفوك)
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تم تزويد هذا السجل من قبل Institut national de la recherche agronomique