Investigating the Changes in the Antioxidant and Enzyme System of Litopenaeus Vannamei during Exposure to Silver Nitrate

BACKGROUND: Oxidative stress is believed to be one of the major causes of tissue damage in aquatic animals exposed to heavy metals. It leads to certain changes in the antioxidant and enzyme system. Given the fact that research on the effect of sub-acute toxicity of silver nitrate in shrimps is not very developed, the present study can be conducive to formulating the international standards of contamination of shrimps with silver nitrate.OBJECTIVES: The present study aimed to evaluate the toxic effects of silver nitrate on the changes in the antioxidant and enzyme systems of hepatopancreas, muscle and gills of Litopenaeus vannamei.METHODS: After acclimatization of shrimps, they were exposed to silver nitrate with concentrations of 0.0084, 0.021, 0.042 and 0.063 mg/L for 21 days. At the end of the experimental period, gill, muscle and hepatopancreas were sampled, and the changes in the antioxidant system (SOD, GPx and GST) and metabolic enzymes (ALT, ALP, AST and LDH) were examined.RESULTS: There was no significant difference in terms of SOD and GST activity in the gill and muscle of the exposed shrimps (P>0.05). However, GPx in treatment 4 increased significantly in gill and muscle while it saw a decrease in hepatopancreas (P<0.05). In hepatopancreas, GST significantly increased in treatments 3 and 4 (P<0.05) whereas SOD did not show any significant changes compared with other treatments (P>0.05). The metabolic enzymes of the muscle did not show any significant differences in any of the treatments (P>0.05), but in gill, the level of ALT in treatment 4 decreased significantly while the levels of AST and LDH in treatment 3 and 4 significantly increased (P<0.05). In hepatopancreas, the activity of ALT in treatments 2 and 4, AST in treatments 3 and 4, and ALP in all treatment except treatment 1 saw significant reduction. Nevertheless, LDH in treatments 3 and 4 had a significant rise (P<0.05).CONCLUSIONS: A significant increase in GST and LDH as well as a significant decrease in GPx and ALT, AST and ALP enzymes in the hepatopancreas revealed that the antioxidant and enzyme system of shrimps is further disturbed with the rise in silver nitrate concentration in the hepatopancreas compared to the gill and muscle.