Molecular and supra-molecular structure of waxy starches developed from cassava (Manihot esculenta Crantz)
2013
Rolland-Sabaté, Agnès | Sanchez, Teresa | Buleon, Alain | Colonna, Paul | Ceballos, Hernán | Zhao, Shan-Shan | Zhang, Peng | Dufour, Dominique | Unité de recherche sur les Biopolymères, Interactions Assemblages (BIA) ; Institut National de la Recherche Agronomique (INRA) | International Center for Tropical Agriculture [Colombie] (CIAT) ; Consultative Group on International Agricultural Research [CGIAR] (CGIAR) | Chinese Academy of Sciences (CAS) | Démarche intégrée pour l'obtention d'aliments de qualité (UMR Qualisud) ; Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad)-Université Montpellier 1 (UM1)-Université Montpellier 2 - Sciences et Techniques (UM2)-Centre international d'études supérieures en sciences agronomiques (Montpellier SupAgro)-Avignon Université (AU)-Université de La Réunion (UR)-Université de Montpellier (UM)-Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro) | Conseil Regional des Pays de la Loire; National Basic Research Program [2010CB126605]; Earmarked Fund for Modern Agro-industry Technology Research System [nycytx-17]; Sino-Swiss Science and Technology Cooperation
International audience
اظهر المزيد [+] اقل [-]إنجليزي. The aim of this work was to characterize the amylopectin of low amylose content cassava starches obtained from transgenesis comparatively with a natural waxy cassava starch (WXN) discovered recently in CIAT (International Center for Tropical Agriculture). Macromolecular features, starch granule morphology, crystallinity and thermal properties of these starches were determined. (M) over bar (w) of amylopectin from the transgenic varieties are lower than WXN. Branched and debranched chain distributions analyses revealed slight differences in the branching degree and structure of these amylopectins, principally on DP 6-9 and DP > 37. For the first time, a deep structural characterization of a series of transgenic lines of waxy cassava was carried out and the link between structural features and the mutated gene expression approached. The transgenesis allows to silenced partially or totally the GBSSI, without changing deeply the starch granule ultrastructure and allows to produce clones with similar amylopectin as parental cassava clone. (C) 2012 Elsevier Ltd. All rights reserved.
اظهر المزيد [+] اقل [-]الكلمات المفتاحية الخاصة بالمكنز الزراعي (أجروفوك)
المعلومات البيبليوغرافية
تم تزويد هذا السجل من قبل Institut national de la recherche agronomique