Comparative transcriptome analysis identifies potentially relevant genes in rubber clones with a high latex yield (Hevea brasiliensis)
2022
Ahmad Sofiman Othman | Mohd Fahmi Abu Bakar
Since the 1950s, the rubber tree (Hevea brasiliensis) has significantly contributed to Malaysia's agricultural economy due to its capability to produce high-value natural rubber. Due to the presence of cis-1,4-polyisoprene, natural rubber exhibits unique properties such as flexibility, high elasticity, and efficient heat dispersion. Cis-1,4-polyisoprene is synthesized via two distinct metabolic pathways: the isoprenoid and rubber biosynthesis. RNA sequencing was performed on 12 specimens from the bark, leaf, and latex tissues of two rubber clones, RRIM 3001 (high latex yield) and RRIM 712 (low latex yield). After quality assessment, these specimens generated a mean of 73,816,244 clean reads. The differential expression analysis revealed that the Acetyl-CoA-Acetyltransferase (AACT) and Diphosphomevalonate Decarboxylase (MVD) genes (via the MVA pathway), as well as the Small Rubber Particle Protein (SRPP) and Rubber Elongation Factor (REF) genes (via the rubber biosynthesis pathway), were overexpressed in the bark, leaf, and latex tissues of RRIM 3001 compared to RRIM 712. The transcription factor Ethylene Response Factor (ERF) was also found to be more highly expressed in RRIM 3001 than in RRIM 712. Five rubber clones with random yield characteristics were used in quantitative amplification analysis to validate AACT, MVD, SRPP, REF, and ERF genes. The quantitative analysis shows that the five genes were consistently expressed with the yield characteristics of the five random rubber clones.
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