Trienoic fatty acids are required to maintain chloroplast function at low temperatures
2000
Routaboul, Jean-Marc, J.-M. | Fischer, Steven F. | Browse, John | Biologie des Semences (LBS) ; Institut National de la Recherche Agronomique (INRA)-Institut National Agronomique Paris-Grignon (INA P-G) | Institute of Biological Chemistry ; Washington State University (WSU) | The U.S. National Science Foundation (grant nos. IBN–9407902 and 0084329) | the Agricultural Research Center, Washington State University. S.F.F. | Training Center under the U.S. Department of Energy (grant no. DE–FG0694ER20160)
International audience
اظهر المزيد [+] اقل [-]إنجليزي. The chloroplast membranes of all higher plants contain very high proportions of trienoic fatty acids. To investigate how these lipid structures are important in photosynthesis, we have generated a triple mutant line of Arabidopsis that contains negligible levels of trienoic fatty acids. For mutant plants grown at 22°C, photosynthetic fluorescence parameters were indistinguishable from wild type at 25°C. Lowering the measurement temperature led to a small decrease in photosynthetic quantum yield,ΦII, in the mutant relative to wild-type controls. These and other results indicate that low temperature has only a small effect on photosynthesis in the short term. However, long-term growth of plants at 4°C resulted in decreases in fluorescence parameters, chlorophyll content, and thylakoid membrane content in triple-mutant plants relative to wild type. Comparisons among different mutant lines indicated that these detrimental effects of growth at 4°C are strongly correlated with trienoic fatty acid content with levels of 16:3 + 18:3, approximately one-third of wild type being sufficient to sustain normal photosynthetic function. In total, our results indicate that trienoic fatty acids are important to ensure the correct biogenesis and maintenance of chloroplasts during growth of plants at low temperatures.
اظهر المزيد [+] اقل [-]الكلمات المفتاحية الخاصة بالمكنز الزراعي (أجروفوك)
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تم تزويد هذا السجل من قبل Institut national de la recherche agronomique