Serum and Muscle Metabolomics for the Prediction of Ultimate pH, a Key Factor for Chicken-Meat Quality
2016
Beauclercq, Stéphane | Nadal-Desbarats, Lydie | Hennequet-Antier, Christelle | Collin, Anne | Tesseraud, Sophie | Bourin, Marie | Le Bihan-Duval, Elisabeth | Berri, Cécile | Unité de Recherches Avicoles (URA) ; Institut National de la Recherche Agronomique (INRA) | Université de Tours (UT) | Institut Technique de l'AVIculture (ITAVI) | CASDAR optiviande
International audience
اظهر المزيد [+] اقل [-]إنجليزي. Variations in muscle glycogen storage are highly correlated with variations in meat ultimate pH (pHu), a key factor for poultry meat quality. A total of two chicken lines were divergently selected on breast pHu to understand the biological basis for variations in meat quality (i.e., the pHu– and the pHu+ lines that are characterized by a 17% difference in muscle glycogen content). The effects of this selection on bird metabolism were investigated by quantifying muscle metabolites by high-resolution NMR (1H and 31P) and serum metabolites by 1H NMR. A total of 20 and 26 discriminating metabolites between the two lines were identified by orthogonal partial least-squares discriminant analysis (OPLS-DA) in the serum and muscle, respectively. There was over-representation of carbohydrate metabolites in the serum and muscle of the pHu– line, consistent with its high level of muscle glycogen. However, the pHu+ line was characterized by markers of oxidative stress and muscle catabolism, probably because of its low level of energy substrates. After OPLS-DA multiblock analysis, a metabolic set of 15 high-confidence biomarkers was identified that could be used to predict the quality of poultry meat after validation on an independent population.
اظهر المزيد [+] اقل [-]الكلمات المفتاحية الخاصة بالمكنز الزراعي (أجروفوك)
المعلومات البيبليوغرافية
تم تزويد هذا السجل من قبل Institut national de la recherche agronomique