Biocontrol of grapevine aerial and root pathogens by Paenibacillus sp strain B2 and paenimyxin in vitro and in planta
2017
Hao, Zhipeng | van Tuinen, Diederik | Wipf, Daniel | Fayolle, Léon | Chataignier, Odile | Li, Xiaolin | Chen, Baodong | Gianinazzi, Silvio | Gianinazzi-Pearson, Vivienne | Adrian, Marielle | Agroécologie [Dijon] ; Institut National de la Recherche Agronomique (INRA)-Université de Bourgogne (UB)-AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement-Université Bourgogne Franche-Comté [COMUE] (UBFC) | Centre National de la Recherche Scientifique (CNRS) | Université Bourgogne Franche-Comté [COMUE] (UBFC) | State Key Laboratory of Urban and Regional Ecology, Research Center for Eco-Environmental Sciences ; Chinese Academy of Sciences [Changchun Branch] (CAS) | AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement | College of Resources and Environmental Sciences ; China Agricultural University (CAU) | Conseil Regional de Bourgogne, France [079201A AO40S3619]; National Natural Science Foundation of China [41571250]; China Scholarship Council
Paenibacillus sp. strain B2, isolated from the mycorrhizosphere of Sorghum bicolor, was previously shown to possess antagonistic activity against fungi and bacteria due to the secretion of the peptide paenimyxin. The effects of strain B2 and paenimyxin were assessed against grapevine pathogens as they potentially represent a non-polluting complement or an alternative biocontrol agent to existing disease management strategies. In this study, the effects of Paenibacillus sp. strain B2 and paenimyxin were analyzed in vitro or in planta on the aerial or root pathogens of grapevine Botrytis cinerea, Plasmopara viticola and Xiphinema index. The bacterium significantly reduced B. cinerea development in vitro, and substantially decreased X. index numbers in soil and gall formation in the root system of grapevine plants. In vitro, paenimyxin inhibited the mycelial growth of B. cinerea, the mobility of the zoospores of P. viticola and X. index activity. Supplying paenimyxin at the time of inoculation of leaf tissue with P. viticola decreased infection sites, compared to application prior to fungal inoculation. Using the P. viticola/grapevine pathosystem, we showed that paenimyxin acts as a biopesticide against the oomycete but does not trigger plant defense. Conversely, Paenibacillus sp. strain B2 can induce the expression of the defense genes CHI (chitinase), PR-3 (proteinase inhibitor) and LOX (lipoxygenase). Altogether, the results obtained highlight the interest of Paenibacillus sp. strain B2 and paenimyxin for the biocontrol of different grapevine diseases.
اظهر المزيد [+] اقل [-]الكلمات المفتاحية الخاصة بالمكنز الزراعي (أجروفوك)
المعلومات البيبليوغرافية
تم تزويد هذا السجل من قبل Institut national de la recherche agronomique