Enhancement of Lytic Activity by Leptin Is Independent From Lipid Rafts in Murine Primary Splenocytes
2017
Collin, Aurore | Noacco, Audrey | Talvas, Jérémie | Caldefie-Chezet, Florence | Vasson, Marie-Paule | Farges, Marie-Chantal | Unité de Nutrition Humaine (UNH) ; Institut National de la Recherche Agronomique (INRA)-Université Clermont Auvergne [2017-2020] (UCA [2017-2020]) | Centre de Recherche en Nutrition Humaine d'Auvergne (CRNH d'Auvergne) | Faculté de Pharmacie | Cancéropôle Lyon Auvergne Rhône-Alpes | Centre Jean Perrin [Clermont-Ferrand] (UNICANCER/CJP) ; UNICANCER
The authors thank Dr. M.G. Kolonin (Center for Stem Cell and Regenerative Medicine, Institute of Molecular Medicine,University of Texas Health Science Center, Houston) for providing the syngeneic EO771 spontaneous mammary adenocarcinoma cell line
اظهر المزيد [+] اقل [-]إنجليزي. Leptin, a pleiotropic adipokine, is known as a regulator of food intake, but it is also involved in inflammation, immunity, cell proliferation, and survival. Leptin receptor is integrated inside cholesterol-rich microdomains called lipid rafts, which, if disrupted or destroyed, could lead to a perturbation of lytic mechanism. Previous studies also reported that leptin could induce membrane remodeling. In this context, we studied the effect of membrane remodeling in lytic activity modulation induced by leptin. Thus, primary mouse splenocytes were incubated with methyl-beta-cyclodextrin (beta-MCD), a lipid rafts disrupting agent, cholesterol, a major component of cell membranes, or ursodeoxycholic acid (UDCA), a membrane stabilizer agent for 1 h. These treatments were followed by splenocyte incubation with leptin (absence, 10 and 100 ng/ml). Unlike beta-MCD or cholesterol, UDCA was able to block leptin lytic induction. This result suggests that leptin increased the lytic activity of primary spleen cells against syngenic EO771 mammary cancer cells independently from lipid rafts but may involve membrane fluidity. Furthermore, natural killer cells were shown to be involved in the splenocyte lytic activity. To our knowledge it is the first publication in primary culture that provides the link between leptin lytic modulation and membrane remodeling
اظهر المزيد [+] اقل [-]الكلمات المفتاحية الخاصة بالمكنز الزراعي (أجروفوك)
المعلومات البيبليوغرافية
تم تزويد هذا السجل من قبل Institut national de la recherche agronomique