Specialization of oleosins in OB dynamics during seed development in Arabidopsis thaliana seeds.
2014
Miquel, Martine | Trigui, Ghassen | d'Andrea, Sabine | Kelemen, Zsolt | Baud, Sebastien, S. | Berger, Adeline | Deruyffelaere, Carine | Trubuil, Alain | Lepiniec, Loic | Dubreucq, Bertrand | Institut Jean-Pierre Bourgin (IJPB) ; Institut National de la Recherche Agronomique (INRA)-AgroParisTech | Unité de recherche Mathématiques et Informatique Appliquées (MIA) ; Institut National de la Recherche Agronomique (INRA)
Oil bodies (OBs) are seed-specific lipid storage organelles that allow accumulation of neutral lipids that sustain plantlet development after the onset of germination. OBs are covered with specific proteins embedded in a single layer of phospholipids. Using fluorescent dyes and confocal microscopy, we monitored the dynamics of OBs in living arabidopsis embryos at different stages of development. Analyses were carried out with different genotypes i.e. the wild type and 3 mutants affected in the accumulation of various oleosins (OLE1, OLE2 and OLE4), three major OB proteins. Image acquisition was followed by a detailed statistical analysis of OB size and distribution during seed development in the 4 dimensions (X, Y, Z, T). Our results indicate that OB size increases sharply during seed maturation, in part by OB fusion, and then decreases until the end of the maturation process. In single, double and triple mutant backgrounds, the size and spatial distribution of OBs are modified, affecting in turn the total lipid content, which suggests that the oleosins studied have specific functions in the dynamics of lipid accumulation.
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