Regulation of enteroendocrine cell respiration by the microbial metabolite hydrogen sulfide
2023
Larraufie, Pierre | Haroun, Kenza | Fleury, Carla | Andriamihaja, Mireille | Blachier, François | Physiologie de la Nutrition et du Comportement Alimentaire (PNCA (UMR 0914)) ; AgroParisTech-Université Paris-Saclay-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE) | MICrobiologie de l'ALImentation au Service de la Santé (MICALIS) ; AgroParisTech-Université Paris-Saclay-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE) | This project was funded by AgroParisTech (Fond d’amorçage), and an award supported by EFSD/NovoNordisk (Rising Star 2020).
International audience
اظهر المزيد [+] اقل [-]إنجليزي. Endocrine functions of the gut are supported by a scattered population of cells, the enteroendocrine cells (EECs). EECs sense their environment to secrete hormones in a regulated manner. Distal EECs are in contact with various microbial compounds including hydrogen sulfide (H 2 S) which modulate cell respiration with potential consequences on EEC physiology. However, the effect of H 2 S on gut hormone secretion remains discussed and the importance of the modulation of cell metabolism on EEC functions remains to be deciphered. The aim of this project was to characterize the metabolic response of EECs to H 2 S and the consequences on GLP-1 secretion. We used cell line models of EECs to assess their capacity to metabolize H 2 S at low concentration and the associated modulation of cell respiration. We confirmed that like what is observed in colonocytes, colonic EEC model, NCI-h716 cell line rapidly metabolizes H 2 S at low concentrations, resulting in transient increased respiration. Higher concentrations of H 2 S inhibited this respiration, with the concentration threshold for inhibition depending on cell density. However, increased or inhibited oxidative respiration had little effect on acute GLP-1 secretion. Overall, we present here a first study showing the EEC capacity to detoxify low concentrations of H 2 S and used this model to acutely address the importance of cell respiration on secretory activity.
اظهر المزيد [+] اقل [-]الكلمات المفتاحية الخاصة بالمكنز الزراعي (أجروفوك)
المعلومات البيبليوغرافية
تم تزويد هذا السجل من قبل Institut national de la recherche agronomique