Next-generation monitoring of aquatic biodiversity using environmental DNA metabarcoding | Nouvelle génération de suivi de la biodiversité aquatique à partir de l'ADN environnemental
2015
Valentini, A. | Taberlet, P. | Miaud, C. | Civade, Raphaël | Herder, J. | Thomsen, P.F. | Bellemain, E. | Besnard, Aurélien | Coissac, E. | Boyer, F. | Gaboriaud, C. | Jean, P. | Poulet, N. | Roset, N. | Copp, G.H. | Geniez, Philippe | Pont, Didier | Argillier, C. | Baudoin, J.M. | Peroux, T. | Crivelli, A.J. | Olivier, A. | Acqueberge, M. | Le Brun, M. | Moller, P.R. | Willerslev, E. | Dejean, T. | Savoie Technolac (Technolac) | Laboratoire d'Ecologie Alpine (LECA) ; Université Joseph Fourier - Grenoble 1 (UJF)-Université Savoie Mont Blanc (USMB [Université de Savoie] [Université de Chambéry])-Centre National de la Recherche Scientifique (CNRS) | Centre d’Ecologie Fonctionnelle et Evolutive (CEFE) ; Université Paul-Valéry - Montpellier 3 (UPVM)-Institut National de la Recherche Agronomique (INRA)-Centre international d'études supérieures en sciences agronomiques (Montpellier SupAgro)-École Pratique des Hautes Études (EPHE) ; Université Paris Sciences et Lettres (PSL)-Université Paris Sciences et Lettres (PSL)-Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD [Occitanie])-Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro) | Hydrosystèmes et Bioprocédés (UR HBAN) ; Institut national de recherche en sciences et technologies pour l'environnement et l'agriculture (IRSTEA) | RAVON ; Ravon | Centre for GeoGenetics ; Natural History Museum of Denmark ; Faculty of Science [Copenhagen] ; University of Copenhagen = Københavns Universitet (UCPH)-University of Copenhagen = Københavns Universitet (UCPH)-Faculty of Science [Copenhagen] ; University of Copenhagen = Københavns Universitet (UCPH)-University of Copenhagen = Københavns Universitet (UCPH) | Office national de l'eau et des milieux aquatiques (ONEMA) | Centre for Environment, Fisheries and Aquaculture Science [Lowestoft] (CEFAS) | Risques, Ecosystèmes, Vulnérabilité, Environnement, Résilience (RECOVER) ; Institut national de recherche en sciences et technologies pour l'environnement et l'agriculture (IRSTEA) | Station Biologique de la Tour du Valat | Agence Centre-Ouest ; Ecosphère | Laboratoire National d’Hydraulique et Environnement (EDF R&D LNHE) ; EDF R&D (EDF R&D) ; EDF (EDF)-EDF (EDF) | Natural History Museum of Denmark ; Faculty of Science [Copenhagen] ; University of Copenhagen = Københavns Universitet (UCPH)-University of Copenhagen = Københavns Universitet (UCPH)
[Departement_IRSTEA]Eaux [TR1_IRSTEA]QUASARE
اظهر المزيد [+] اقل [-]International audience
اظهر المزيد [+] اقل [-]إنجليزي. Global biodiversity in freshwater and the oceans is declining at high rates. Reliable tools for assessing and monitoring aquatic biodiversity, especially for rare and secretive species, are important for efficient and timely management. Recent advances in DNA sequencing have provided a new tool for species detection from DNA present in the environment. In this study, we tested whether an environmental DNA (eDNA) metabarcoding approach, using water samples, can be used for addressing significant questions in ecology and conservation. Two key aquatic vertebrate groups were targeted: amphibians and bony fish. The reliability of this method was cautiously validated in silico, invitro and insitu. When compared with traditional surveys or historical data, eDNA metabarcoding showed a much better detection probability overall. For amphibians, the detection probability with eDNA metabarcoding was 0.97 (CI=0.90-0.99) vs. 0.58 (CI=0.50-0.63) for traditional surveys. For fish, in 89% of the studied sites, the number of taxa detected using the eDNA metabarcoding approach was higher or identical to the number detected using traditional methods. We argue that the proposed DNA-based approach has the potential to become the next-generation tool for ecological studies and standardized biodiversity monitoring in a wide range of aquatic ecosystems. see also the Perspective by Hoffmann, Schubert and Calvignac-Spencer.
اظهر المزيد [+] اقل [-]الكلمات المفتاحية الخاصة بالمكنز الزراعي (أجروفوك)
المعلومات البيبليوغرافية
تم تزويد هذا السجل من قبل Institut national de la recherche agronomique