Molecular and immunological characterization of three strains of <em>Anaplasma marginale</em> grown in cultured tick cells.
2015
Lis, Katarzyna | Fernández de Mera, Isabel G. | Popara, Marina | Cabezas-Cruz, Alejandro | Ayllon, Nieves | Zweygarth, Erich | Passos, Lygia M.F. | Broniszewska, Marzena | Villar, Margarita | Kocan, Katherine M. | Ribeiro, Mucio F.B. | Pfister, Kurt | de La Fuente, Jose | Comparative Tropical Medicine and Parasitology ; Ludwig-Maximilians University [Munich] (LMU) | SaBio ; Instituto de Investigación en Recursos Cinegéticos (IREC) | Centre d’Infection et d’Immunité de Lille - INSERM U 1019 - UMR 9017 - UMR 8204 (CIIL) ; Institut Pasteur de Lille ; Pasteur Network (Réseau International des Instituts Pasteur)-Pasteur Network (Réseau International des Instituts Pasteur)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Lille-Centre Hospitalier Régional Universitaire [CHU Lille] (CHRU Lille)-Centre National de la Recherche Scientifique (CNRS) | Department of Veterinary Tropical Diseases, Faculty of Veterinary Science ; University of Pretoria [South Africa] | Universidade Federal de Minas Gerais = Federal University of Minas Gerais [Belo Horizonte, Brazil] (UFMG) | Department of Veterinary Pathobiology, Center for Veterinary Health Sciences ; Oklahoma State University [Stillwater] (OSU) | ITN programme (EU Grant No. 238511); BFU2011-23896; EU FP7 ANTIGONE project number 278976; MEC Spain.
Contribution: Phylogenetic and sequence analysis.
اظهر المزيد [+] اقل [-]International audience
اظهر المزيد [+] اقل [-]إنجليزي. Anaplasma marginale is an economically important tick-borne pathogen of cattle that causes bovine anaplasmosis. A wide range of geographic strains of A. marginale have been isolated from cattle, several of which have been characterized using genomics and proteomics. While many of these strains have been propagated in tick lines, comparative analyses after propagation in tick cells have not been reported. The overall purpose of this research therefore was to compare the degree of conservation of selected genes after propagation in tick cell culture among A. marginale strains from the U.S. (the Virginia strain) and Brazil (UFMG1 and UFMG2 strains). The genes studied herein included those which encode the proteins HSP70 and SODB involved in heat shock and stress responses, respectively, and two genes that encode major surface proteins MSP4 and MSP5. Strain identities were first confirmed by sequencing the tandem repeats of the msp1a gene which encodes for the adhesin, MSP1a. The results of these studies demonstrated that the genes encoding for both stress response and heat shock proteins were highly conserved among the three A. marginale strains. Antibodies specific for MSP4, MSP5, SODB and HSP70 proteins were used to further characterize the A. marginale strains, and they reacted with all of these strains propagated in tick cell culture, providing further evidence for antigenic conservation. Although antigenic differences were not found among the three A. marginale strains, multi-locus sequence analysis (MLSA) performed with nucleotide sequences of these genes demonstrated that the A. marginale Brazilian and U.S. strains fall in different clades. These results showed that phylogenetically distant strains of A. marginale are antigenically conserved, even after several in vitro passages, supporting the use of some of the above conserved proteins as candidates for universal vaccines.
اظهر المزيد [+] اقل [-]المعلومات البيبليوغرافية
تم تزويد هذا السجل من قبل Institut national de la recherche agronomique