Fungal secretomics of ascomycete fungi for biotechnological applications
2016
Couturier, Marie | Navarro, David | Favel, Anne | Haon, Mireille | Lechat, C. | Lesage-Meessen, Laurence | Chevret, Didier | Lombard, Vincent | Henrissat, Bernard | Berrin, Jean-Guy | Biodiversité et Biotechnologie Fongiques (BBF) ; Institut National de la Recherche Agronomique (INRA)-Aix Marseille Université (AMU)-École Centrale de Marseille (ECM) | ASCOfrance | MICrobiologie de l'ALImentation au Service de la Santé (MICALIS) ; Institut National de la Recherche Agronomique (INRA)-AgroParisTech | Architecture et fonction des macromolécules biologiques (AFMB) ; Institut National de la Recherche Agronomique (INRA)-Aix Marseille Université (AMU)-Centre National de la Recherche Scientifique (CNRS) | Centre National de la Recherche Scientifique (CNRS) | Dept Biol Sci ; University of Alabama [Birmingham] (UAB) | E-TRICEL ANR-07-BIOE-006
The enzymatic degradation of plant biomass is of growing interest for the development of a sustainable bio-based industry. Ascomycete fungi, which degrade complex and recalcitrant plant polymers, secrete enzymes acting on the different components of plant cell wall (cellulose, hemicellulose and lignin). In this study, we present proteomic analyses of enzyme cocktails (secretomes) produced by five strains of Ascomycota (Aspergillus wentii, Aspergillus niger, Aspergillus niger, Neocosmospora haematococca, Penicillium variabile) from different geographical origins. Expert annotation of enzymes secreted revealed a large array of carbohydrate-active enzymes targeting plant cell wall polysaccharides. This study reveals that systematic proteomic analyses of fungal secretomes can contribute to the phenotyping of fungal strains from different geographical origins.
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تم تزويد هذا السجل من قبل Institut national de la recherche agronomique