Role of whey components in the kinetics and thermodynamics of β-Lactoglobulin unfolding and aggregation
2016
Petit, Jérémy | Moreau, Anne | Ronce, Gilles | Debreyne, Pascal | Bouvier, Laurent | Blanpain-Avet, Alain | Jeantet, Romain | Delaplace, Guillaume | Science et Technologie du Lait et de l'Oeuf (STLO) ; Institut National de la Recherche Agronomique (INRA)-AGROCAMPUS OUEST | Laboratoire d'Ingénierie des Biomolécules (LIBio) ; Université de Lorraine (UL) | This work, constituting a part of the PERE (Procédé Electromagnétique de Réduction d’Encrassement, i.e. Eletromagnetic device for the reduction of milk fouling) project, has been funded by INRA Transfert within the framework of the 2010 patent valorisation campaign.
The optimisation of dairy unit operations involving heat transfer requires the control of fouling and aggregationphenomena following the denaturation of thermosensitive proteins, in particular β-lactoglobulin (β-lg). This study intends to give a better view of the influence of whey components (whey proteins, lactose, and minerals such as calcium) on β-lg denaturation through a combined kinetic and thermodynamic approach. β-lg denaturation in model solutions of increasing complexity (pure β-lg solution, whey protein solution, and two model wheys differing in mineral content) was characterised at temperatures ranging from 64.5 to 98 °C by following the evolution of soluble β-lg concentration with HPLC. It was demonstrated that whatever the model solutioncomposition, a two-step mechanism (unfolding followed by aggregation) of 1.5-order kinetics could be adopted to describe β-lg denaturation reaction, as the temperature dependence of the denaturation reaction rate was properly fitted by Arrhenius equation. The dependency of kinetic and thermodynamic parameters on solution composition indicated that the presence of whey proteins enhanced β-lg aggregation, whereas lactose showed a small protective effect against β-lg unfolding. Additionally, minerals, especially calcium, tended to stabilise β-lg native state while increasing β-lg aggregation rates. However, at high mineral content, calcium influence could be hindered or even reversed, presumably owing to a lower bioavailability due to complexation withanions such as inorganic phosphates
اظهر المزيد [+] اقل [-]الكلمات المفتاحية الخاصة بالمكنز الزراعي (أجروفوك)
المعلومات البيبليوغرافية
تم تزويد هذا السجل من قبل Institut national de la recherche agronomique