Early postovulatory aging reveals the first proteomic markers of egg quality in pikeperch
2024
Nynca, Joanna | Bobe, Julien | Król, Jarosław | Ljubobratović, Uroš | Palińska-Żarska, Katarzyna | Malinowska, Agata | Świderska, Bianka | Żarski, Daniel | Polska Akademia Nauk = Polish Academy of Sciences = Académie polonaise des sciences (PAN) | Laboratoire de Physiologie et Génomique des Poissons = Fish Physiology and Genomics Institute (LPGP) ; Structure Fédérative de Recherche en Biologie et Santé de Rennes (Biosit : Biologie - Santé - Innovation Technologique)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE) | National Inland Fisheries Research Institute | Research Centre of Fisheries and Aquaculture | This work was funded by the National Science Center, Poland (NCN) [grant number 2016/22/M/NZ9/00590].
International audience
اظهر المزيد [+] اقل [-]إنجليزي. This study explored the molecular mechanisms underlying egg quality deterioration due to in vivo postovulatory aging in pikeperch (Sander lucioperca L.), a key species in aquaculture. We employed tandem mass tag (TMT) peptide labeling coupled with LC–MS/MS quantitative proteomics to analyze eggs collected at various postovulation intervals. Our research revealed four distinct proteomic markers (Gins4, Atrx, DnaJB14, and Mrpl10) that are differentially expressed in response to early aging, shedding light on their potential roles in DNA replication, chromatin organization, protein folding, and mitochondrial function. The study confirmed that eggs maintain morphological integrity up to 5 h postovulation but exhibit compromised fertilization capacity, underscoring the importance of timely egg utilization in aquaculture practices. These findings enhance the understanding of egg aging at the molecular level, offering insights for improving reproductive success and larval quality in pikeperch aquaculture. The data are available via ProteomeXchange with the identifier PXD048349
اظهر المزيد [+] اقل [-]الكلمات المفتاحية الخاصة بالمكنز الزراعي (أجروفوك)
المعلومات البيبليوغرافية
تم تزويد هذا السجل من قبل Institut national de la recherche agronomique