Prevention of Bacterial Biofilms Formation on Urinary Catheter by Selected Plant Extracts
2015
Nwinyi, Obinna C
In this study, we investigated the feasibility of using Psidium guajava, Mangifera indica and Ocimum gratissimum leaf extracts in preventing Escherichia coli biofilm formation. The plants extractions were done with methanol under cold extraction. The various concentrations 5.0, 10.0 and 20.0 mg/mL were used to coat 63 catheters under mild heat from water bath. Biofilm formation on the catheter was induced using cultures of E. coli. Biofilm formation was evaluated using aerobic plate count and turbidity at 600 nm. From the obtained results, Psidium guajava, Mangifera indica and Ocimum gratissimum delayed the onset of biofilm formation for a week. Ocimum gratissimum coated catheter had the highest inhibitory effect at 5.0, 10.0 and 20.0 mg/mL with bacterial count ranging from 2.2×105 - 7.0×104 and 5.7×105 - 3.7×105 for 120 and 128 hrs respectively. The Psidium guajava coated catheter had the lowest inhibitory effect at 5.0, 10.0 and 20.0 mg/mL, with bacterial count ranging between 4.3×105 - 1.9 ×103 and 7.7×105- 3.8×105 for 120 and 128 hrs respectively. Despite the antimicrobial activities, the differences in the activity of these plant extracts were statistically not significant (P < 0.05).
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