Experimental Parameters Influence the Observed Antimicrobial Response of Oak Wood (Quercus petraea)
2020
Munir, Muhammad Tanveer | Pailhories, Hélène | Eveillard, Matthieu | Irle, Mark | Aviat, Florence | Federighi, Michel, M. | Belloncle, Christophe | Laboratoire Innovation Matériau Bois Habitat Apprentissage [Nantes] (LIMBHA) ; Ecole Supérieure du Bois (ESB) | Hémodynamique, Interaction Fibrose et Invasivité tumorales Hépatiques (HIFIH) ; Université d'Angers (UA) | Centre Hospitalier Universitaire d'Angers (CHU Angers) | Centre de Recherche en Cancérologie et Immunologie Nantes-Angers (CRCINA) ; Université d'Angers (UA)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Centre Hospitalier Universitaire de Nantes = Nantes University Hospital (CHU Nantes)-Université de Nantes - UFR de Médecine et des Techniques Médicales (UFR MEDECINE) ; Université de Nantes (UN)-Université de Nantes (UN) | YouR ResearcH-Bio-Scientific [Le Landreau, France] | SECurité des ALIments et Microbiologie (SECALIM) ; École nationale vétérinaire, agroalimentaire et de l'alimentation Nantes-Atlantique (ONIRIS)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE) | ANR-16-IDEX-0007,NExT (I-SITE),NExT (I-SITE)(2016)
International audience
اظهر المزيد [+] اقل [-]إنجليزي. The present investigation aimed to utilize a direct wood disc diffusion method to study the influence of plane of cutting, cutting method, sterilization method, and origin of tree on the antimicrobial activity of wood material. Six oak wood trees (Quercus petraea) were collected from 3 different locations in France. They were cut into 4 mm thick slices with either transverse (RT), tangential (LT) or radial (LR) faces. Round discs (diameter 9.95 ± 0.1 mm) were cut from the slices via a laser machine or a manual punch machine, and were sterilized with gamma irradiation (25 kGy) or autoclaving (121 °C). The antimicrobial activity of wood was tested using a direct diffusion method against Staphylococcus aureus and Acinetobacter baumannii isolates. The zone of inhibition around the wooden disc was recorded following the recommendations used for antibiotics tests. The results showed that S. aureus was more susceptible than A. baumannii, to the chemicals that diffused from the wood. The transverse face discs exhibited higher antimicrobial activity. Samples that had been sterilized by autoclaving showed significantly (p < 0.05) lower antimicrobial activity, whereas the cutting method and origin of tree did not influence the antimicrobial activity of wood material. Therefore, the choice of sterilization method and cutting planes must be taken into account while studying and interpreting the antibacterial properties of wood material
اظهر المزيد [+] اقل [-]الكلمات المفتاحية الخاصة بالمكنز الزراعي (أجروفوك)
المعلومات البيبليوغرافية
تم تزويد هذا السجل من قبل Institut national de la recherche agronomique