The future of biotic indices in the ecogenomic era: Integrating (e)DNA metabarcoding in biological assessment of aquatic ecosystems
2018
Pawlowski, Jan | Kelly-Quinn, Mary | Altermatt, Florian | Apothéloz-Perret-Gentil, Laure | Beja, Pedro | Boggero, Angela | Borja, Angel | Bouchez, Agnes | Cordier, Tristan | Domaizon, Isabelle | Feio, Maria João | Filipe, Ana Filipa | Fornaroli, Riccardo | Graf, Wolfram | Herder, Jelger | van Der Hoorn, Berry | Iwan Jones, J. | Sagova-Mareckova, Marketa | Moritz, Christian | Barquin, Jose | Piggott, Jeremy J. | Pinna, Maurizio | Rimet, Frédéric | Rinkevich, Buki | Sousa-Santos, Carla | Specchia, Valeria | Trobajo, Rosa | Vasselon, Valentin | Vitecek, Simon | Zimmerman, Jonas | Weigand, Alexander | Leese, Florian | Kahlert, Maria | Université de Genève = University of Geneva (UNIGE) | School of Biology and Environmental Science ; University College Dublin [Dublin] (UCD) | Department of Aquatic Ecology ; Swiss Federal Institute of Aquatic Science and Technology [Dübendorf] (EAWAG)-IBZ | Universität Zürich [Zürich] = University of Zurich (UZH) | Universidade do Porto = University of Porto | Universidade de Lisboa = University of Lisbon = Université de Lisbonne (ULISBOA) | LifeWatch Italy ; LifeWatch ERIC | National Research Council of Italy | Consiglio Nazionale delle Ricerche (CNR) | Marine Research Division, ; AZTI - Centro de Investigación Marina y Alimentaria | Centre Alpin de Recherche sur les Réseaux Trophiques et Ecosystèmes Limniques (CARRTEL) ; Institut National de la Recherche Agronomique (INRA)-Université Savoie Mont Blanc (USMB [Université de Savoie] [Université de Chambéry]) | Universidade de Coimbra | Università degli Studi di Milano = University of Milan (UNIMI) | Institute of Hydrobiology | RAVON ; Ravon | National Biodiversity Centre | Queen Mary University of London (QMUL) | Crop research institute | ARGE Limnologie ; Partenaires INRAE | Universidad de Cantabria [Santander] = University of Cantabria [Spain] = Université de Cantabrie [Espagne] (UC / UniCan) | University of Dublin | Department of Zoology ; Auburn University (AU) | Università del Salento = University of Salento [Lecce] | Israel Oceanographic and Limnological Research (IOLR) | Instituto Superior de Psicologia Aplicada | Institut de Recerca i Tecnologia Agroalimentàries = Institute of Agrifood Research and Technology (IRTA) | Universität Wien = University of Vienna | Senckenberg Research Institute and Natural History Museum [Frankfurt] ; Senckenberg – Leibniz Institution for Biodiversity and Earth System Research - Senckenberg Gesellschaft für Naturforschung ; Leibniz Association-Leibniz Association | Senckenberg Museum [Frankfurt] | Free University of Berlin (FU) | Universität Duisburg-Essen = University of Duisburg-Essen [Essen] | Muséum national d'Histoire naturelle (MNHN) | Swedish University of Agricultural Sciences = Sveriges lantbruksuniversitet (SLU) | European Union CA15219;Swiss National Sciences Foundation 313003A_159709; Swedish Agency for Marine and Water Management (SwAM); Swiss National Science Foundation PP00P3_150698 31003A_173074; EDP Biodiversity Chair; FRESHING Project - FCT;COMPETE PTDC/AAG-MAA/2261/2014-POCI-01-0145-FEDER-356 016824; German Federal Ministry for Education and Research (BMBF)01LI1501E 01Li1501K; Italian Ministry of University and Research (MIUR);Interreg-ADRION CUP C69H18000250007;Ministry of National Infrastructures, Energy and Water Resources, Israel; French Biodiversity Agency (AFB);Ministry of Education, Youth and Sports of the Czech Republic LTC17075 | European Project: 668981,H2020-WIDESPREAD-2014-2015,H2020-WIDESPREAD-2014-2,EnvMetaGen(2015)
International audience
اظهر المزيد [+] اقل [-]إنجليزي. The bioassessment of aquatic ecosystems is currently based on various biotic indices that use the occurrence and/or abundance of selected taxonomic groups to define ecological status. These conventional indices have some limitations, often related to difficulties in morphological identification of bioindicator taxa. Recent development of DNA barcoding and metabarcoding could potentially alleviate some of these limitations, by using DNA sequences instead of morphology to identify organisms and to characterize a given ecosystem. In this paper, we review the structure of conventional biotic indices, and we present the results of pilot metabarcoding studies using environmental DNA to infer biotic indices. We discuss the main advantages and pitfalls of metabarcoding approaches to assess parameters such as richness, abundance, taxonomic composition and species ecological values, to be used for calculation of biotic indices. We present some future developments to fully exploit the potential of metabarcoding data and improve the accuracy and precision of their analysis. We also propose some recommendations for the future integration of DNA metabarcoding to routine biomonitoring programs.
اظهر المزيد [+] اقل [-]الكلمات المفتاحية الخاصة بالمكنز الزراعي (أجروفوك)
المعلومات البيبليوغرافية
تم تزويد هذا السجل من قبل Institut national de la recherche agronomique