Modulation of blackspot seabream (Pagellus bogaraveo) intermediary metabolic pathways by dispensable amino acids
2010
Figueiredo-Silva, A.C. | Corraze, Geneviève | Kaushik, Sadasivam, S. | Peleteiro, J.B. | Valente, L.M.P. | Nutrition, Aquaculture et Génomique (NUAGE) ; Institut National de la Recherche Agronomique (INRA)-Université Sciences et Technologies - Bordeaux 1 (UB)-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER) | Centro de Investigação Marinha e Ambiental (CIMA) ; Universidade do Algarve (UAlg) | Instituto Español de Oceanografía - Spanish Institute of Oceanography (IEO) ; Consejo Superior de Investigaciones Cientificas [España] = Spanish National Research Council [Spain] (CSIC)
International audience
اظهر المزيد [+] اقل [-]إنجليزي. The objective of the present work is to investigate the main metabolic pathways by which dispensable amino acids (DAA) are diverted towards lipid formation in blackspot seabream. For that purpose, a control diet was formulated to contain 45% of crude protein (7.2 g N/100 g dry matter) mainly supplied by fish meal (45P). In two other diets, 22.2% of the dietary nitrogen (1.6 g N/100 g dry matter) was replaced by an equivalent amount of nitrogen provided by two different mixtures of DAA: alanine and serine (diet AS) or aspartic and glutamic acid (diet AG). A fourth diet (diet 35P) only containing 35% of crude protein (5.6 g N/100 g dry matter) was included in order to analyze the possible additive effects of DAA. Compared to fish fed diet 35P, blackspot seabream appear to make a more efficient use of the nitrogen provided by alanine and serine than that provided by aspartic and glutamic acids in terms of growth. Contrary to fish fed AG, fish fed AS attained similar specific FAS activities as 45P fed fish, suggesting a further role of alanine and serine on this lipogenic pathway. Dietary nitrogen reduction (45P vs. 35P) or its replacement by a mixture of aspartic and glutamic acids (diet AG) were shown to up-regulate phosphoenolpyruvate carboxykinase (PEPCK) but without, however, any effect on plasma glucose levels. Dietary nitrogen level and nature seems to exert a complex regulation on energetic pathways through the gluconeogenesis/tricarboxylic acids cycle interaction.
اظهر المزيد [+] اقل [-]الكلمات المفتاحية الخاصة بالمكنز الزراعي (أجروفوك)
المعلومات البيبليوغرافية
تم تزويد هذا السجل من قبل Institut national de la recherche agronomique