Molecular Investigation of Small Ruminant Abortions Using a 10-Plex HRM-qPCR Technique: A Novel Approach in Routine Diagnostics
2024
Gouvias, Ioannis | Lysitsas, Marios | Batsidis, Apostolos | Malefaki, Sonia | Bitchava, Dimitra | Tsara, Anna | Nickovic, Emilija | Bouzalas, Ilias | Malissiova, Eleni | Guatteo, Raphaël | Valiakos, George | University of Thessaly [Lamia] | University of Ioannina | University of Patras | Veterinary lab vet in progress plus ; Partenaires INRAE | University of Belgrade [Belgrade] | Hellenic Agricultural Organization Demeter (HAO Demeter) | Biologie, Epidémiologie et analyse de risque en Santé Animale (BIOEPAR) ; École nationale vétérinaire, agroalimentaire et de l'alimentation Nantes-Atlantique (ONIRIS)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE) | This research was partially funded by CEVA HELLAS, Grant number 7652 (University of Thessaly).
International audience
اظهر المزيد [+] اقل [-]إنجليزي. The objective of this study was to apply and preliminarily evaluate a High-Resolution Melting (HRM) analysis technique coupled with qPCR, that allows the simultaneous detection of 10 different ruminant abortogenic pathogens, for investigating abortions in sheep and goats throughout Greece. A total of 264 ovine and caprine vaginal swabs were obtained the week following the abortion from aborted females and analyzed using a commercially available kit (ID Gene™ Ruminant Abortion Multiplex HRM, Innovative Diagnostics). Results indicated a high prevalence of Coxiella burnetii and Chlamydophila spp., which were detected in 48.9% and 42.4% of the vaginal swabs, respectively. Results for these most commonly detected pathogens were compared with those of a well-established commercial qPCR kit, with near-perfect agreement. Toxoplasma gondii, Salmonella spp., Brucella spp., Anaplasma phagocytophilum, Campylobacter fetus, and Neospora caninum were also identified, the two latter reported for the first time in the country in small ruminants. Mixed infections occurred in 35.6% of the animals examined. This technique allows for the simultaneous detection of many abortogenic pathogens in an accurate and cost-effective assay. Detection of uncommon or not previously reported pathogens in various cases indicates that their role in ovine and caprine abortions may be underestimated.
اظهر المزيد [+] اقل [-]الكلمات المفتاحية الخاصة بالمكنز الزراعي (أجروفوك)
المعلومات البيبليوغرافية
تم تزويد هذا السجل من قبل Institut national de la recherche agronomique