Use of volatile compound metabolic signatures in poultry tissues to back-trace dietary exposure to xenobiotics
2011
Engel, Erwan | Ratel, Jérémy | Berge, Philippe | Khummueng, Weeraya | Feidt, Cyril | Roudaut, Brigitte | Bizec, Bruno Le | Qualité des Produits Animaux (QuaPA) ; Institut National de la Recherche Agronomique (INRA) | Prince of Songkla University (PSU) | Unité de Recherches Animal et Fonctionnalités des Produits Animaux (URAFPA) ; Institut National de la Recherche Agronomique (INRA)-Université de Lorraine (UL) | Agence nationale de sécurité sanitaire de l'alimentation, de l'environnement et du travail (ANSES) | École nationale vétérinaire, agroalimentaire et de l'alimentation Nantes-Atlantique (ONIRIS)
International audience
اظهر المزيد [+] اقل [-]إنجليزي. We investigated the feasibility of using volatile compound signatures of liver in poultry to detect previous dietary exposure to different types of xenobiotic. Six groups of broiler chickens were fed a similar diet either non-contaminated or contaminated with polychlorinated dibenzo-p-dioxins/-furans (PCDD/Fs), polychlorinated biphenyls (PCBs), polybrominated diphenyl ethers (PBDEs), polycyclic aromatic hydrocarbons (PAHs) or coccidiostats. The liver of each chicken was analysed by solid-phase microextraction - mass spectrometry (SPME-MS) for volatile compound metabolic signature and by gas chromatography - high resolution mass spectrometry (GC-HRMS), gas chromatography - tandem mass spectrometry (GC-MS/MS) and liquid chromatography - tandem mass spectrometry (LC-MS/MS) to quantify xenobiotic residues. The results show that the volatile compound metabolic signature could clearly differentiate the non-contaminated chickens from those contaminated with PBDEs, PAHs or coccidiostats. The results for PAHs showed a clear metabolic response in the liver although these rapidly metabolized xenobiotics are undetectable in this organ by the targeted reference analytical method. However, the rough metabolic signature obtained by SPME-MS did not enable us to evidence previous exposure to slowly metabolized compounds such as PCDD/Fs and PCBs, the residues of which are clearly detected by targeted reference methods.
اظهر المزيد [+] اقل [-]الكلمات المفتاحية الخاصة بالمكنز الزراعي (أجروفوك)
المعلومات البيبليوغرافية
تم تزويد هذا السجل من قبل Institut national de la recherche agronomique
