A Capsule-Based Model for Immature Hard Tick Stages Infestation on Laboratory Mice
2020
Mateos Hernandez, Lourdes | Rakotobe, Sabine | Defaye, Baptiste | Cabezas Cruz, Alejandro | Simo, Ladislav | Biologie moléculaire et immunologie parasitaires et fongiques (BIPAR) ; École nationale vétérinaire d'Alfort (ENVA)-Laboratoire de santé animale, sites de Maisons-Alfort et de Normandie ; Agence nationale de sécurité sanitaire de l'alimentation, de l'environnement et du travail (ANSES)-Agence nationale de sécurité sanitaire de l'alimentation, de l'environnement et du travail (ANSES)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE) | Faculté de Pharmacie [Limoges] ; Université de Limoges (UNILIM) | Laboratoire « Sciences pour l’Environnement » (UMR CNRS 6134 SPE) (SPE) ; Centre National de la Recherche Scientifique (CNRS)-Università di Corsica Pasquale Paoli [Université de Corse Pascal Paoli] | DIM One Health - Région Île-de-France (Acronym of the project: NeuroPaTick)
International audience
اظهر المزيد [+] اقل [-]إنجليزي. Ticks are obligatory blood feeding parasites at all stages of development (except eggs) and are recognized as vectors of various pathogens. The use of mouse models in tick research is critical for understanding their biology and tick-host-pathogen interactions. Here we demonstrate a non-laborious technique for the feeding of immature stages of hard ticks on laboratory mice. The benefit of the method is its simplicity, short duration, and the ability to monitor or collect ticks at different time points of an experiment. In addition, the technique allows attachment of two individual capsules on the same mouse, which is beneficial for a variety of experiments where two different groups of ticks are required to feed on the same animal. The non-irritating and flexible capsule is made from easily accessible materials and minimizes the discomfort of the experimental animals. Furthermore, euthanasia is not necessary, mice recover completely after the experiment and are available for re-use.
اظهر المزيد [+] اقل [-]المعلومات البيبليوغرافية
تم تزويد هذا السجل من قبل Institut national de la recherche agronomique