Synchoronised physical, chemical and microbial analysis of wastewater from University of Limpopo food- court

Thesis (M.Sc. (Chemistry)) -- University of Limpopo, 2021

The present research work deals with the study of some of the important physicochemical parameters of restaurant wastewater effluents collected from Mankweng business centre along the University of Limpopo road. Studies revealed that restaurants, food preparation facilities, engineering, paper mill, fine chemical, dyes, paint, pharmaceutical, petrochemical and textile industries are some of the major industries responsible for polluting the aquatic environments. This research attempts to review the physical, chemical, and microbial analysis of wastewater from food service establishments (FSEs) and extraction of fats, oil, and grease (FOG) and its characterization using different analytical techniques. The elemental analysis revealed that the concentration of elements such as zinc, lead and manganese were 6.44 mg/L, 0.02 mg/L and 0.53 mg/L, respectively. The concentration of these elements was above the required South African National Standards 241 (SANS241) limit, that is, ≤5mg/L, ≤0.01mg/L and ≤0.4mg/L for Zn, Pb and Mn, respectively. The elevated concentrations may be due to corrosion, acidic pH found in some samples and high organic load. High organic content in wastewater influenced the production of oxygen by microorganisms in-order to degrade the amount of waste in the wastewater and this has led to high chemical oxygen demand (COD) level ranging from 1784-7196 mg/L and biochemical oxygen demand (BOD5) from 1101.32 to 3222.32 mg/L. The study has demonstrated that the wastewater samples are contaminated with faecal coliforms because the concentration of microbes Escherichia Coli (E.coli) with a Most Probable Number (MPN) >200.5 in most samples, P. aeruginosa, Pseudomonas spp. were too numerous to count (TNC) and total coliforms at the highest concentration of 9.1x103 CFU/ml which was influenced by change in pH of the wastewater, the FOG content consists of both triglycerides and acrolein. The overall results highlight the discharge of highly polluted wastewater effluent, these FSE have resulted in pollution of nearby rivers thereby affecting the growth of vegetation and aquatic life. The results of the present investigation point out the need to implement common objectives, compatible policies, and programmes for improvement in the FSEs wastewater treatment methods.

National Research Foundation and Sasol-Inzalo Foundation