Assessment of microbial plankton diversity as an ecological indicator in the NW Mediterranean coast

12 pages, 7 figures, 2 tables, supplementary material https://doi.org/10.1016/j.marpolbul.2020.111691

High-throughput sequencing of microbial assemblages has been proposed as an alternative methodology to the traditional ones used in marine monitoring and environmental assessment. Here, we evaluated pico- and nanoplankton diversity as ecological indicators in NW Mediterranean coastal waters by comparing their diversity in samples subjected to varying degrees of continental pressures. Using metabarcoding of the 16S and 18S rRNA genes, we explored whether alphadiversity indices, abundance of Operational Taxonomic Units and taxonomic groups (and their ratios) provide information on the ecological quality of coastal waters. Our results revealed that only eukaryotic diversity metrics and a limited number of prokaryotic and eukaryotic taxa displayed potential in assessing continental influences in our surveyed area, resulting thus in a restrained potential of microbial plankton diversity as an ecological indicator. Therefore, incorporating microbial plankton diversity in environmental assessment could not always result in a significant improvement of current marine monitoring strategies

This work is an outcome of the DEVOTES (Development of innovative tools for understanding marine biodiversity and assessing GEnS) project, funded by the European Union (grant agreement n° 308392), and the Spanish grant GRADIENTS (Fine-scale structure of cross-shore gradients along the Mediterranean coast, CTM2012-39479-C02)

With the funding support of the ‘Severo Ochoa Centre of Excellence’ accreditation (CEX2019-000928-S), of the Spanish Research Agency (AEI)

Peer reviewed