Genomics and biochemical analyses reveal a metabolon key to β-L-ODAP biosynthesis in Lathyrus sativus
2023
Edwards, Anne | Njaci, Isaac | Sarkar, Abhimanyu | Jiang, Zhouqian | Kaithakottil, Gemy George | Moore, Christopher | Cheema, Jitender | Stevenson, Clare E.M. | Rejzek, Martin | Novák, Petr | Vigouroux, Marielle | Vickers, Martin | Wouters, Roland H.M. | Paajanen, Pirita | Steuernagel, Burkhard | Moore, Jonathan D. | Higgins, Janet | Swarbreck, David | Martens, Stefan | Kim, Colin Y. | Weng, Jing-Ke | Mundree, Sagadevan | Kilian, Benjamin | Kumar, Shiv | Loose, Matt | Yant, Levi | Macas, Jiří | Wang, Trevor L. | Martin, Cathie | Emmrich, Peter M.F.
Grass pea ( Lathyrus sativus L.) is a rich source of protein cultivated as an insurance crop in Ethiopia, Eritrea, India, Bangladesh, and Nepal. Its resilience to both drought and flooding makes it a promising crop for ensuring food security in a changing climate. The lack of genetic resources and the crop’s association with the disease neurolathyrism have limited the cultivation of grass pea. Here, we present an annotated, long read-based assembly of the 6.5 Gbp L. sativus genome. Using this genome sequence, we have elucidated the biosynthetic pathway leading to the formation of the neurotoxin, β-L-oxalyl-2,3-diaminopropionic acid (β-L-ODAP). The final reaction of the pathway depends on an interaction between L. sativus acyl-activating enzyme 3 (LsAAE3) and a BAHD-acyltransferase (LsBOS) that form a metabolon activated by CoA to produce β-L-ODAP. This provides valuable insight into the best approaches for developing varieties which produce substantially less toxin.
اظهر المزيد [+] اقل [-]الكلمات المفتاحية الخاصة بالمكنز الزراعي (أجروفوك)
المعلومات البيبليوغرافية
تم تزويد هذا السجل من قبل International Livestock Research Institute