Characterization of a set of variable number of tandem repeat markers conserved in Bovidae
1991
Georges, Michel | Gunawardana, A. | Threadgill, D. | Lathrop, M. | Olsaker, I. | Mishra, A. | Sargeant, L. | Steele, M. | Terry, C. H. | Zhao, X. | Holm, T. | Fries, R. | Womack, J.
إنجليزي. peer reviewed
اظهر المزيد [+] اقل [-]إنجليزي. Screening purpose-built libraries with minisatellite probes, we have isolated 36 bovine variable number of tandem repeat markers (VNTRs) characterized by a mean heterozygosity of 59.3 within the American Holstein breed. Matching probabilities and exclusion powers were estimated by Monte-Carlo simulation, showing that the top 5 to 10 markers could be used as a very efficient DNA-based system for individual identification and paternity diagnosis. The isolated VNTR systems should contribute significantly to the establishment of a bovine primary DNA marker map. Linkage analysis, use of somatic cell hybrids, and in situ hybridization demonstrate that these bovine VNTRs are scattered throughout the bovine genome, without evidence for proterminal confinement as in the human, and that at least some of them are organized as clusters. Moreover, Southern blot analysis and in situ hybridization demonstrate conservation of sequence and map location of minisatellites within Bovidae
اظهر المزيد [+] اقل [-]الكلمات المفتاحية الخاصة بالمكنز الزراعي (أجروفوك)
المعلومات البيبليوغرافية
تم تزويد هذا السجل من قبل University of Liège