Enhancing Nitrogen Fertilisation Efficiency by Developing Novel Nitrification Inhibitors for a Greener Agriculture
2024 | 2026
Yildirim, Sibel Cansu | Brüggemann, Nicolas | Knief, Claudia
Nitrogen fertilization in agriculture has serious environmental consequences, including production of the greenhouse gas nitrous oxide (N<sub>2</sub>O), pollution of groundwater with nitrate (NO<sub>3</sub><sup>-</sup>), and river eutrophication. Nitrogen use efficiency can be increased by amending fertilizers with inhibitors to slow microbial nitrification processes, which transform ammonia to NO<sub>3</sub><sup>-</sup>. Unfortunately, commercial inhibitors have failed to perform reliably across various agroecosystems for reasons not well understood. Using a combination of bacterial studies and soil incubations, we demonstrate here that 4-methyl-1-(prop-2-yn-1-yl)-1H-1,2,3-triazole (MPT) exhibits superior nitrification inhibitory properties. Unlike the commercial reversible inhibitors, MPT acts as a mechanistic, irreversible inhibitor of the key enzyme ammonia monooxygenase, enabling effective retention of ammonium (NH<sub>4</sub><sup>+</sup>) and suppression of NO<sub>3</sub><sup>-</sup> and N<sub>2</sub>O production over 21 days in several agricultural soils with pH values ranging from 4.7 to 7.5. A bacterial viability stain and a suite of freshwater and terrestrial ecotoxicity tests did not indicate any acute or chronic toxicity. Real-time quantitative polymerase chain reaction (qPCR) analysis revealed an enhanced inhibitory effect of MPT on both ammonia-oxidizing bacteria and archaea. Thus, MPT outperforms currently available nitrification inhibitors and has great potential for broad application in various agricultural settings.
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