Induction of neutralising antibodies by virus-like particles harbouring surface proteins from highly pathogenic H5N1 and H7N1 influenza viruses.
2006
Szécsi, Judit | Boson, Bertrand | Johnsson, Per | Dupeyrot-Lacas, Pia | Matrosovich, Mikhail | Klenk, Hans-Dieter | Klatzmann, David | Volchkov, Viktor | François-Loïc, Cosset | Virologie humaine ; École normale supérieure de Lyon (ENS de Lyon) ; Université de Lyon-Université de Lyon-Institut National de la Santé et de la Recherche Médicale (INSERM) | BioSciences Lyon-Gerland (BLG) ; École normale supérieure de Lyon (ENS de Lyon) ; Université de Lyon-Université de Lyon-Institut National de la Recherche Agronomique (INRA)-Université Claude Bernard Lyon 1 (UCBL) ; Université de Lyon-Hospices Civils de Lyon (HCL)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS) | Epixis SA [Lyon, France] | Institut fur Virologie ; Universitatsklinikum Giessen und Marburg | Biologie et thérapeutique des pathologies immunitaires (BTPI) ; Université Pierre et Marie Curie - Paris 6 (UPMC)-Centre National de la Recherche Scientifique (CNRS) | This work was supported by the European Community (contract LSHBCT- 2004-005246 "COMPUVAC"), the Région Rhône-Alpes (FITT 2005) and the Agence Nationale pour la Recherche (ANR "MIME").
SUMMARY: There is an urgent need to develop novel approaches to vaccination against the emerging, highly pathogenic avian influenza viruses. Here, we engineered influenza viral-like particles (Flu-VLPs) derived from retroviral core particles that mimic the properties of the viral surface of two highly pathogenic influenza viruses of either H7N1 or H5N1 antigenic subtype. We demonstrate that, upon recovery of viral RNAs from a field strain, one can easily generate expression vectors that encode the HA, NA and M2 surface proteins of either virus and prepare high-titre Flu-VLPs. We characterise these Flu-VLPs incorporating the HA, NA and M2 proteins and we show that they induce high-titre neutralising antibodies in mice.
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تم تزويد هذا السجل من قبل Institut national de la recherche agronomique