Endogenous viral elements are targeted by RNA silencing pathways in banana
2024
Duroy, Pierre‐Olivier | Seguin, Jonathan | Ravel, Sébastien | Rajendran, Rajeswaran | Laboureau, Nathalie | Salmon, Frédéric | Delos, Jean-Marie | Pooggin, Mikhail | Iskra‐Caruana, Marie‐Line | Chabannes, Matthieu | Plant Health Institute of Montpellier (UMR PHIM) ; Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad)-Institut de Recherche pour le Développement (IRD)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Institut Agro Montpellier ; Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Université de Montpellier (UM) | Zurich Basel Plant Science Center ; Universität Zürich [Zürich] = University of Zurich (UZH)-Université de Bâle = University of Basel = Basel Universität (Unibas) | Amélioration génétique et adaptation des plantes méditerranéennes et tropicales (UMR AGAP) ; Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Institut Agro Montpellier ; Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Université de Montpellier (UM) | Direction Générale Déléguée à la Recherche et à la Stratégie (Cirad-Dgdrs) ; Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad) | This work was supported by a grant from CIRAD and Region Languedoc Roussillon for the PhD grant of PO Duroy and the European Cooperation in Science and Technology (COST) action FA0806. | South Green Platform (http://www.southgreen.fr, Montpellier, France
All the datasets and scripts have been uploaded on the open-source CIRAD dataverse. These data can be accessed using the links provided: BPO-59: https://doi.org/10.18167/DVN1/9K4J0D. BPO-60: https://doi.org/10.18167/DVN1/5LFLWU. BPO-64: https://doi.org/10.18167/DVN1/WJPM4N. BPO-66: https://doi.org/10.18167/DVN1/TPDEX8. GWT-1: https://doi.org/10.18167/DVN1/PNUURS. GWT-2: https://doi.org/10.18167/DVN1/XIOTAO. GWT-3: https://doi.org/10.18167/DVN1/EVHGJO. GWT-4: https://doi.org/10.18167/DVN1/TYWQ0R. GWT-5: https://doi.org/10.18167/DVN1/0JUVIK. GWT-6: https://doi.org/10.18167/DVN1/IHRVQS. GWT-7: https://doi.org/10.18167/DVN1/XF2A3E. GWT-9: https://doi.org/10.18167/DVN1/9IHYTJ. GWT-10: https://doi.org/10.18167/DVN1/JHKGIO. GWT-11: https://doi.org/10.18167/DVN1/LB5HTS. GWT-12: https://doi.org/10.18167/DVN1/YKFVR1. GWT-13: https://doi.org/10.18167/DVN1/59AVAI. GWT-14: https://doi.org/10.18167/DVN1/ZNXDNL. GWT-15: https://doi.org/10.18167/DVN1/NPRFXF. GWT-16: https://doi.org/10.18167/DVN1/IX2M3J. GWT-17: https://doi.org/10.18167/DVN1/HPQU43. GWT-18: https://doi.org/10.18167/DVN1/L4LBY7. GWT-19: https://doi.org/10.18167/DVN1/YTRCBN. Scripts for RNA-Seq and sRNA analyses: https://doi.org/10.18167/DVN1/K1OKGY.
اظهر المزيد [+] اقل [-]International audience
اظهر المزيد [+] اقل [-]إنجليزي. . Endogenous banana streak virus (eBSV) integrants derived from three distinct species, present in Musa balbisiana (B) but not Musa acuminata (A) banana genomes are able to reconstitute functional episomal viruses causing banana streak disease in interspecific triploid AAB banana hybrids but not in the diploid (BB) parent line, which harbours identical eBSV loci. Here, we investigated the regulation of these eBSV.. In-depth characterization of siRNAs, transcripts and methylation derived from eBSV using Illumina and bisulfite sequencing were carried out on eBSV-free Musa acuminata AAA plants and BB or AAB banana plants with eBSV.. eBSV loci produce low-abundance transcripts covering most of the viral sequence and generate predominantly 24-nt siRNAs. siRNA accumulation is restricted to duplicated and inverted viral sequences present in eBSV. Both siRNA-accumulating and nonaccumulating sequences of eBSV in BB plants are heavily methylated in all three CG, CHG and CHH contexts.. Our data suggest that eBSVs are controlled at the epigenetic level in BB diploids. This regulation not only prevents their awakening and systemic infection of the plant but is also probably involved in the inherent resistance of the BB plants to mealybug-transmitted viral infection. These findings are thus of relevance to other plant resources hosting integrated viruses.
اظهر المزيد [+] اقل [-]الكلمات المفتاحية الخاصة بالمكنز الزراعي (أجروفوك)
المعلومات البيبليوغرافية
تم تزويد هذا السجل من قبل Institut national de la recherche agronomique