Ex situ and in situ investigation of protein/exopolysaccharide complex in Porphyridium cruentum biomass resuspension
2019
Tran, Thierry | Lafarge, Céline | Winckler, Pascale | Pradelles, Rémi | Cayot, Nathalie | Loupiac, Camille | Procédés Alimentaires et Microbiologiques [Dijon] (PAM) ; Université de Bourgogne (UB)-AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement-Université Bourgogne Franche-Comté [COMUE] (UBFC) | Dispositif Inter-régional d'Imagerie Cellulaire [Dijon] (DImaCell) ; Procédés Alimentaires et Microbiologiques (PAM) ; Université de Bourgogne (UB)-AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement-Université de Bourgogne (UB)-AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement-Ingénierie et biologie cellulaire et tissulaire (IBCT (ex IFR133)) ; Centre Hospitalier Régional Universitaire de Besançon (CHRU Besançon)-Etablissement français du sang [Bourgogne-Franche-Comté] (EFS BFC)-Université de Franche-Comté (UFC) ; Université Bourgogne Franche-Comté [COMUE] (UBFC)-Université Bourgogne Franche-Comté [COMUE] (UBFC)-Centre Hospitalier Régional Universitaire de Besançon (CHRU Besançon)-Etablissement français du sang [Bourgogne-Franche-Comté] (EFS BFC)-Université de Franche-Comté (UFC) ; Université Bourgogne Franche-Comté [COMUE] (UBFC)-Université Bourgogne Franche-Comté [COMUE] (UBFC)-Agroécologie [Dijon] ; Université de Bourgogne (UB)-AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement-Université Bourgogne Franche-Comté [COMUE] (UBFC)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE) | Agroécologie [Dijon] ; Institut National de la Recherche Agronomique (INRA)-Université de Bourgogne (UB)-AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement-Université Bourgogne Franche-Comté [COMUE] (UBFC) | Microphyt | Regional Council of Bourgogne - Franche ComteRegion Bourgogne-Franche-Comte; Fonds Europeens de Developpement Regional (FEDER)European Union (EU)
International audience
اظهر المزيد [+] اقل [-]إنجليزي. During the extraction processes of B-phycoerythrin from P. cruentum, the possibility of the occurrence of extraction-inhibiting B-phycoerythrin/exopolysaccharide (EPS) complexes was highlighted. The use of confocal laser scanning microscopy allowed the observation of the cells including their sheath and their chloroplast, the bound EPS matrix, and extracellular vesicles involving phycobiliproteins and EPS in P. cruentum biomass resuspensions. The observations were performed using autofluorescence and fluorescence marking.pH variations showed more impact than ionic strength increase induced by NaCl addition on the B-phycoerythrin content and the organization of vesicles. Namely, agglomeration of the vesicles could be observed at pH 8.5 without NaCl addition. With addition of 1 M NaCl, this phenomenon was prevented but the vesicles were not visibly dissociated for any pH value. These results point towards a precipitate rather than a coacervate, which are expected to have narrow stability conditions regarding pH and ionic strength.In an attempt to dissolve or break the vesicles and the extracellular matrix, successive macerations in water and use of high pressure homogenizer process in one-step or two-step treatments were applied to the resuspensions. The successive macerations could free the cells and the vesicles from the extracellular matrix, whereas the homogenizer treatment disrupted the extracellular matrix, the vesicle and a part of the cells. In our case, only the successive macerations showed a positive extraction since the high pressure treatment caused unwanted denaturation.It is concluded that more experiments in model conditions could help understand the nature of the interactions between phycobiliproteins and EPS leading to the formation of the vesicles.
اظهر المزيد [+] اقل [-]الكلمات المفتاحية الخاصة بالمكنز الزراعي (أجروفوك)
المعلومات البيبليوغرافية
تم تزويد هذا السجل من قبل Institut national de la recherche agronomique