Anaplasma phagocytophilum in passerines from Ile-de-France region (France), genetic characterization of variants by groEL, ankA and MLST typing
2024
Rouxel, Clotilde | Biologie moléculaire et immunologie parasitaires et fongiques (BIPAR) ; École nationale vétérinaire d'Alfort (ENVA)-Laboratoire de santé animale, sites de Maisons-Alfort et de Normandie ; Agence nationale de sécurité sanitaire de l'alimentation, de l'environnement et du travail (ANSES)-Agence nationale de sécurité sanitaire de l'alimentation, de l'environnement et du travail (ANSES)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE) | Biologie moléculaire et immunologie parasitaires et fongiques (BIPAR) ; École nationale vétérinaire d'Alfort (ENVA)-Institut National de la Recherche Agronomique (INRA)-Laboratoire de santé animale, sites de Maisons-Alfort et de Normandie ; Agence nationale de sécurité sanitaire de l'alimentation, de l'environnement et du travail (ANSES)-Agence nationale de sécurité sanitaire de l'alimentation, de l'environnement et du travail (ANSES)-Université Paris-Est Créteil Val-de-Marne - Paris 12 (UPEC UP12) | Adaptation Biologique et Vieillissement = Biological Adaptation and Ageing (B2A-IBPS) ; Institut National de la Santé et de la Recherche Médicale (INSERM)-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS)-Institut de Biologie Paris Seine (IBPS) ; Institut National de la Santé et de la Recherche Médicale (INSERM)-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS) | Adaptation Biologique et Vieillissement = Biological Adaptation and Ageing (B2A) ; Université Pierre et Marie Curie - Paris 6 (UPMC)-Institut de Biologie Paris Seine (IBPS) ; Université Pierre et Marie Curie - Paris 6 (UPMC)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS) | Alfort National Veterinary School (EnvA) | French Agency for Food, Environmental and Occupational Health Safety (ANSES) | French National Research Institute for Agriculture, Food and the Environment (INRAE) | French Government's Investment for the Future program as a Laboratory of Excellence in "Integrative Biology of Emerging Infectious Diseases"
International audience
اظهر المزيد [+] اقل [-]إنجليزي. Anaplasma phagocytophilum (Aph) is a strict intracellular bacterium transmitted by ticks of the genus Ixodes. It causes granulocytic anaplasmosis (GA) in humans and various species of domestic mammals (dogs, cattle, sheep...) also called tick-borne fever (TBF) in ruminants. Contrary to TBF which is particularly prevalent in Europe and is the cause of significant economic losses, human GA is mainly found in the USA. Aph is characterized by high genetic diversity and complex epidemiological cycles, as it is also found in many wild animals (deer, rodents, birds...). Domestic species such as horses and dogs, as well as wild species such as wild boar, foxes and hedgehogs, harbor strains that are suspected of being zoonotic. Conversely, deer, rodents (voles) and shrews, as well as birds apparently, appear to harbor strains that are genetically distant from those that affect humans. Birds play an important role in the maintenance and spread of pathogens. Aph DNA has been identified in avian hosts in Europe, with prevalence rates ranging from 0% to 33.8%. However, few epidemiologic studies have been conducted on birds and Aph. Our project therefore aimed to investigating the presence of Aph in birds from urban and peri-urban areas of Ile-de-France and neighboring departments, particularly in passerines. Liver, spleen and skin samples from 680 passerines were tested for the presence of Aph DNA by qPCR targeting the msp2 gene. Three blackbirds (Turdus merula) were tested positive for Aph DNA. This corresponds to a detection rate of 0.4 % in all the birds tested and 3.3 % in the blackbirds. In a second phase, a phylogenetic study was performed targeting the groEL and ankA genes and using MLST (multilocus sequence typing). These results support the existence of an epidemiological cycle specific to birds, in France, in agreement with what is found in other European countries.
اظهر المزيد [+] اقل [-]الكلمات المفتاحية الخاصة بالمكنز الزراعي (أجروفوك)
المعلومات البيبليوغرافية
تم تزويد هذا السجل من قبل Institut national de la recherche agronomique