Alterations of the gut microbiome of largemouth bronze gudgeon (Coreius guichenoti) suffering from furunculosis
2016
Li, Tongtong | Long, Meng | Ji, Cheng | Shen, Zhixin | Gatesoupe, Francois-Joël | Zhang, Xujie | Zhang, Qianqian | Zhang, Lanli | Zhao, Yuanli | Liu, Xinhua | Li, Aihua | State Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology ; Chinese Academy of Sciences [Beijing] (CAS) | Key Laboratory of Environmental and Applied Microbiology, Environmental Microbiology Key Laboratory of Sichuan Province, Chengdu Institute of Biology ; Chinese Academy of Sciences (CAS) | Center for Circadian Clocks ; Soochow University | Qinghai Provincial Fishery Environmental Monitoring Center | Nutrition, Métabolisme, Aquaculture (NuMéA) ; Institut National de la Recherche Agronomique (INRA)-Université de Pau et des Pays de l'Adour (UPPA) | This work was supported by grants from FEBL project (2011FBZ26), Jiangsu Provincial Department of Science and Technology project D2015-11 and the Natural Science Foundation of China (No. 30670112 and 31070112)
International audience
اظهر المزيد [+] اقل [-]إنجليزي. High-throughput sequencing was applied to compare the intestinal microbiota in largemouth bronze gudgeon either healthy or affected by furunculosis. Proteobacteria, Actinobacteria, Tenericutes, Firmicutes and Bacteroidetes were detected as the predominant bacterial phyla in the gut of both diseased and healthy fish. The abundance of Proteobacteria differed significantly between the two groups of fish, mainly due to the overwhelming prevalence of Aeromonas in the diseased fish (81% ± 17%), while the genus was unevenly spread among the apparently healthy fish (33% ± 33%). The bacterial diversity in the intestine of diseased fish was markedly lower than in healthy fish. Analysis revealed the significant dissimilarity between the gut microbiota of diseased and healthy fish. The bacterial profiles in the gut were further characterized with the 28 phylotypes that were shared by the two groups. In diseased fish, two shared OTUs (OTU0001 and OTU0013) were closely related to Aeromonas salmonicida, their total proportion exceeding 70% of the sequences in diseased fish, while averaging 5.2% ± 4.6% in the healthy fish. This result suggested the presence of healthy carriers of pathogenic A. salmonicida among the farmed fish, and the gut appeared as a probable infection source for furunculosis in largemouth bronze gudgeon
اظهر المزيد [+] اقل [-]الكلمات المفتاحية الخاصة بالمكنز الزراعي (أجروفوك)
المعلومات البيبليوغرافية
تم تزويد هذا السجل من قبل Institut national de la recherche agronomique