Multilocus variable number of tandem repeat analysis reveals multiple introductions in spain of xanthomonas arboricola pv. pruni, the causal agent of bacterial spot disease of stone fruits and almond
2016
Lopez-Soriano, Pablo | Boyer, Karine | Cesbron, Denis | Clara Morente, Maria | Penalver, Javier | Palacio-Bielsa, Ana | Vernière, Christian | Lopez, Maria M. | Pruvost, Olivier | Instituto Valenciano de Investigaciones Agrarias - Institut Valencià d'Investigacions Agraries - Valencian Institute for agricultural Research (IVIA) | Peuplements végétaux et bioagresseurs en milieu tropical (UMR PVBMT) ; Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad)-Institut de Recherche pour le Développement (IRD)-Institut National de la Recherche Agronomique (INRA)-Université de La Réunion (UR) | Institut de Recherche en Horticulture et Semences (IRHS) ; Université d'Angers (UA)-Institut National de la Recherche Agronomique (INRA)-AGROCAMPUS OUEST | Instituto Agroalimentario de Aragón (IA2) ; Universidad de Zaragoza = University of Zaragoza [Saragossa University] = Université de Saragosse-Centro de Investigación y Tecnología Agroalimentaria de Aragón (CITA) | Biologie et Génétique des Interactions Plante-Parasite (UMR BGPI) ; Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad)-Institut National de la Recherche Agronomique (INRA)-Centre international d'études supérieures en sciences agronomiques (Montpellier SupAgro)-Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro) | Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad) | Instituto Nacional de Tecnologia Agraria y Alimentaria [RTA-2011-00140-C03-01]; Euphresco project [266505-FP7-ERANET Euphresco II]; European Social Fund; European Regional Development Fund; Conseil Regional de La Reunion; Centre de Cooperation Internationale en Recherche Agronomique pour le Developpement (CIRAD); FPI-INIA grant; Instituto Nacional de Tecnologia Agraria y Alimentaria; AIP Bioressources taxonomic project
Xanthomonas arboricola pv. pruni is the causal agent of the bacterial spot disease of stone fruits, almond and some ornamental Prunus species. In Spain it was first detected in 2002 and since then, several outbreaks have occurred in different regions affecting mainly Japanese plum, peach and almond, both in commercial orchards and nurseries. As the origin of the introduction(s) was unknown, we have assessed the genetic diversity of 239 X. arboricola pv. pruni strains collected from 11 Spanish provinces from 2002 to 2013 and 25 reference strains from international collections. We have developed an optimized multilocus variable number of tandem repeat analysis (MLVA) scheme targeting 18 microsatellites and five minisatellites. A high discriminatory power was achieved since almost 50% of the Spanish strains were distinguishable, confirming the usefulness of this genotyping technique at small spatio-temporal scales. Spanish strains grouped in 18 genetic clusters (conservatively delineated so that each cluster contained haplotype networks linked by up to quadruple-locus variations). Furthermore, pairwise comparisons among populations from different provinces showed a strong genetic differentiation. Our results suggest multiple introductions of this pathogen in Spain and redistribution through contaminated nursery propagative plant material.
اظهر المزيد [+] اقل [-]الكلمات المفتاحية الخاصة بالمكنز الزراعي (أجروفوك)
المعلومات البيبليوغرافية
تم تزويد هذا السجل من قبل Institut national de la recherche agronomique