Immunoprofiling of peripheral blood from infectious bronchitis virus vaccinated MHC-B chicken lines – Monocyte MHC-II expression as a potential correlate of protection
2019
Larsen, Frederik | Bed’hom, Bertrand | Naghizadeh, Mohammad | Kjaerup, Rikke | Zohari, Siamak | Dalgaard, Tina | Institut de Systématique, Evolution, Biodiversité (ISYEB) ; Muséum national d'Histoire naturelle (MNHN)-École Pratique des Hautes Études (EPHE) ; Université Paris Sciences et Lettres (PSL)-Université Paris Sciences et Lettres (PSL)-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS)-Université des Antilles (UA) | Génétique Animale et Biologie Intégrative (GABI) ; Institut National de la Recherche Agronomique (INRA)-AgroParisTech | Aarhus University [Aarhus] | Statens Veterinarmedicinska Anstalt (SVA) | Danish Council for Independent Research [6111-00304B]; Fjerkrsaafgiftsfonden [FAF2017010]
International audience
اظهر المزيد [+] اقل [-]إنجليزي. Vaccination programs are implemented in poultry farms to limit outbreaks and spread of infectious bronchitis virus (IBV), which is a substantial economic burden in the poultry industry. Immune correlates, used to predict vaccine efficacy, have proved difficult to find for IBV-vaccine-induced protection. To find correlates of IBV-vaccine-induced protection, hence, we employed a flow cytometric assay to quantify peripheral leucocyte subsets and expression of cell surface markers of six different non-vaccinated and vaccinated Major Histocompatibility Complex (MHC) haplotypes. Non-vaccinated and vaccinated MHC haplotypes presented differential leucocyte composition and IBV viral load. A strong effect of MHC-B, but not vaccination, on several leucocyte subsets resulted in positive correlations with IBV viral load based on MHC haplotype ranking. In addition, a strong effect of MHC-B and vaccination on monocyte MHC-II expression showed that animals with highest monocyte MHC-II expression had weakest vaccine-induced protection. In conclusion, we found several interesting MHC-B related immune correlates of protection and that flow cytometric analysis can be employed to study correlates of IBV-vaccine-induced protection.
اظهر المزيد [+] اقل [-]الكلمات المفتاحية الخاصة بالمكنز الزراعي (أجروفوك)
المعلومات البيبليوغرافية
تم تزويد هذا السجل من قبل Institut national de la recherche agronomique