The use of electroporation in developmental biology
2017
Gosse, C. | Zhao, X. | Migeotte, I. | Suárez-Boomgaard, D. | Hue, Isabelle | Degrelle, S. | Perea-Gomez, A. | Mazari, E. | Laboratoire de photonique et de nanostructures (LPN) ; Centre National de la Recherche Scientifique (CNRS) | Institut de Recherche Interdisciplinaire en Biologie Humaine et moléculaire = Insitute of Interdisciplinary Research [Bruxelles, Belgique] (IRIBHM) ; Faculté de Médecine [Bruxelles] (ULB) ; Université libre de Bruxelles (ULB)-Université libre de Bruxelles (ULB) | Biologie du Développement et Reproduction (BDR) ; École nationale vétérinaire d'Alfort (ENVA)-Institut National de la Recherche Agronomique (INRA) | Université Paris-Saclay | Physiopathologie et Pharmacotoxicologie Placentaire Humaine (U1139) ; Université Paris Descartes - Paris 5 (UPD5)-Institut National de la Santé et de la Recherche Médicale (INSERM) | Université Sorbonne Paris Cité (USPC) | Université Paris Descartes - Paris 5 (UPD5) | Institut de Biologie Valrose (IBV) ; Université Nice Sophia Antipolis (1965 - 2019) (UNS)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Université Côte d'Azur (UniCA) | U 1091 ; Institut National de la Santé et de la Recherche Médicale (INSERM) | Université de Nice Sophia-Antipolis (UNSA) | University of Tokyo [Tokyo] = Tōkyō teikoku daigaku (UTokyo) | "ElectroMice" (CNRS PI: Physique Chimie Biologie 2008); "Electro- TagMam" (Cnano IdF 2011); “ ElectroDev " (PHC Tournesol 2014); "RhoGTPases and collective migration" (FNRS MIS 2013)
Chapter 139-1
اظهر المزيد [+] اقل [-]إنجليزي. During the formation of a complex organism, cells divide, die, migrate, and differentiate. Biologists have established tools to observe those phenomena but also to change their course, which subsequently enables to infer causal relationships between various events occurring in different cell groups. More precisely, present approaches mostly rely on modifications of gene expression. For instance, cells are labeled with fluorescent proteins and tracked within the embryo, molecular signals are switched on and off to perturb regulatory pathways. Importantly, in all those experiments, the exogenous genetic material must be delivered at the right place and with the appropriate timing: requirements that can both be fulfilled by electroporation. After 15 years of constant refinement, this technique has now superseded methods like viral infection, microinjection, and lipofection. Applications encompass a large number of model organisms, targeted anatomical structures, and molecular biology techniques.
اظهر المزيد [+] اقل [-]الكلمات المفتاحية الخاصة بالمكنز الزراعي (أجروفوك)
المعلومات البيبليوغرافية
تم تزويد هذا السجل من قبل Institut national de la recherche agronomique