Molecular Diagnostics of Ebola Patient Samples by Institut Pasteur de Dakar Mobile Laboratory in Guinea 2014-2016
2020 | 2019
Faye, Oumar | Diagne, Cheikh Tidiane | Diallo, Amadou | Meyer, Emily | Soropogui, Barre | Fall, Gamou | Fall, Cheikh | Magassouba, N'Faly | Koivogui, Lamine | Keita, Sakoba | Loucoubar, Cheikh | Diop, Mamadou | Weidmann, Manfred | Faye, Ousmane | Sall, Amadou Alpha | Okware, Samuel Ikwaras | DFID Department for International Development | Institut Pasteur de Dakar | Institut Pasteur de Dakar | Institut Pasteur de Dakar | Institut Pasteur de Dakar | Laboratory for Hemorrhagic Fevers of Guinea | Institut Pasteur de Dakar | Institut Pasteur de Dakar | Laboratory for Hemorrhagic Fevers of Guinea | National Institute of Public Health Guinea (Guinea NPHI) | Ministry of Health & Public Hygiene (Guinea) | Institut Pasteur de Dakar | Institut Pasteur de Dakar | Institute of Aquaculture | Institut Pasteur de Dakar | Institut Pasteur de Dakar | 0000-0002-7063-7491
As part of the laboratory response to the Ebola virus outbreak in Guinea, the Institut Pasteur de Dakar mobile laboratory (IPD-ML) was set up in Donka hospital from 2014 to 2016. EBOV suspected samples collected at Ebola Treatment Centers (ETC) and from community deaths were sent daily to IPD-ML. Analysis was performed using dried oligonucleotide mixes for real-time RT-PCR designed for field diagnostic. From March 2014 to May 2015, a total of 6055 patient samples suspected for EBOV collected from seven regions of Guinea were tested by real-time RT-PCR. These patients’ clinical included serum samples (n = 2537 samples) and swabs (n = 3518 samples) with positivity rates of 36.74 and 6.88% respectively. Females were significantly more affected than males with positivity rates of 22.39 and 17.22% respectively (p-value = 5.721e-7). All age groups were exposed to the virus with significant difference (p-value
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